Li G D, Lorenzen J, Hansmann M L
Department of Pathology, West China University of Medical Sciences, Chengdu.
Zhonghua Bing Li Xue Za Zhi. 1994 Aug;23(4):211-4.
Using PCR method and family specific primers for T-cell receptor gamma chain V gene, the T-cell colonalities of 12 cases of AILD-TCL were analyzed. The results showed monoclonal DNA amplification patterns of TCR gamma in all 12 cases (13 specimens), whereas the DNA from normal peripheral lymphocytes and 2 samples of atypical lymphoid hyperplasia showed polyclonal rearrangement patterns. Our results suggest that AILD-TCL is a monoclonal proliferative disorder originating from T-cell. The PCR method and family specific primers could successfully amplicate the template DNA from paraffin sections, so it is a sensitive, simple and rapid method for routine hematopathologic studies.
采用聚合酶链反应（PCR）方法及针对T细胞受体γ链V基因的家族特异性引物，对12例AILD-TCL患者的T细胞克隆性进行了分析。结果显示，12例患者（13份标本）的TCRγ均呈现单克隆DNA扩增模式，而来自正常外周淋巴细胞及2份非典型淋巴组织增生标本的DNA则呈现多克隆重排模式。我们的结果提示，AILD-TCL是一种起源于T细胞的单克隆增殖性疾病。PCR方法及家族特异性引物能够成功扩增石蜡切片中的模板DNA，因此是常规血液病理学研究中一种敏感、简便且快速的方法。
