Zhao E F, Zhou M Q, Fu C Y
General Hospital of Chinese PLA, Beijing.
Zhonghua Zhong Liu Za Zhi. 1994 Jul;16(4):273-6.
We examined the cytotoxic activities of recombinant human tumor necrosis factor (rHTNF-alpha) and five chemotherapeutic agents, CTX, 5-Fu, VCR, DDP, KSM, against two human ovarian cancer cell lines, OVCAR3 and CAOV3, using the MTT assay. The results showed that cytotoxicities of rHTNF-alpha at 5 x 10-5 x 10(4) u/ml against OVCAR3 cell line for 24 h exposure were from 14.2 +/- 6.8% to 67.2 +/- 3.0%, and those against CAOV3 cell line were from 8.2 +/- 4.3% to 60.9 +/- 1.3%. The cytotoxic effects of all five chemotherapeutic agents against the two cell lines were much lower than that of rHTNF-alpha. Further, we studied the combined anticancer potential of rHTNF-alpha with chemotherapeutic agents against the two cell lines. Various degrees of synergism in cytotoxicities of DDP or KSM in combination with rHTNF-alpha were observed. The cytotoxic effect of rHTNF-alpha on CAOV3 cell were also morphologically observed under phase contrast and electron microscope. Based on experiment in vitro, the in vivo anticancer activity of rHTNF-alpha alone or in combination with KSM was examined against human ovarian cancer OVCAR3 subcutaneously transplanted in nude mice. After 8 weeks of treatment, a statistically significant difference of mean tumor volume was found between the control group and groups that received rHTNF-alpha or rHTNF-alpha plus KSM (P < 0.01).
我们使用MTT法检测了重组人肿瘤坏死因子（rHTNF-α）和五种化疗药物（CTX、5-Fu、VCR、DDP、KSM）对两种人卵巢癌细胞系OVCAR3和CAOV3的细胞毒活性。结果显示，rHTNF-α在5×10⁻⁵至5×10⁴ U/ml浓度下作用于OVCAR3细胞系24小时的细胞毒性为14.2%±6.8%至67.2%±3.0%，作用于CAOV3细胞系的细胞毒性为8.2%±4.3%至60.9%±1.3%。所有五种化疗药物对这两种细胞系的细胞毒性作用均远低于rHTNF-α。此外，我们研究了rHTNF-α与化疗药物联合对这两种细胞系的抗癌潜力。观察到DDP或KSM与rHTNF-α联合使用时在细胞毒性方面有不同程度的协同作用。还通过相差显微镜和电子显微镜对rHTNF-α对CAOV3细胞的细胞毒性作用进行了形态学观察。基于体外实验，检测了rHTNF-α单独或与KSM联合对裸鼠皮下移植的人卵巢癌OVCAR3的体内抗癌活性。治疗8周后，对照组与接受rHTNF-α或rHTNF-α加KSM的组之间在平均肿瘤体积上存在统计学显著差异（P<0.01）。
