[重组人肿瘤坏死因子（rHTNF）单独及与化疗药物联合对人卵巢癌细胞的细胞毒性]
[Cytotoxicity of recombinant human tumor necrosis factor (rHTNF) alone and in combination with chemotherapeutic agents on human ovarian cancer cells].
作者信息
Zhao E F
机构信息
General Hospital of PLA, Beijing.
出版信息
Zhonghua Fu Chan Ke Za Zhi. 1993 Mar;28(3):153-6, 188.
The cytotoxic activities of recombinant human tumor necrosis factor (rHTNF) and five chemotherapeutic agents, CTX, 5-FU, VCR, DDP, KSM, against two human ovarian cancer cell lines, OVCAR3 and CAOV3, using the MTT assay were studied. The result showed that cytotoxicities of rHTNF at 5 x 10(4)-5 x 10(7) U/L against CAOV3 cell line for 24h exposure were from 14.2% +/- 6.8% to 67.2% +/- 3.0%, and that against CAOV3 cell line were from 8.2% +/- 4.3% to 60.9% +/- 1.3%. The cytotoxic effects of all five chemotherapeutic agents against the two cell lines were much lower than that of rHTNF. Various degrees of synergistic enhancement cytotoxicities of DDP or KSM in combination with rHTNF were assessed. Especially, distinct synergistic effects of a low dose, 50kU/L, or rHTNF with nearly all concentration (10(-4)-10(-1) mg/L) of KSM were obtained on the two cell lines.
采用MTT法研究了重组人肿瘤坏死因子（rHTNF）及5种化疗药物（CTX、5-FU、VCR、DDP、KSM）对两种人卵巢癌细胞系OVCAR3和CAOV3的细胞毒活性。结果显示，rHTNF在5×10⁴ - 5×10⁷ U/L浓度下作用于CAOV3细胞系24小时，细胞毒性为14.2%±6.8%至67.2%±3.0%；作用于CAOV3细胞系时，细胞毒性为8.2%±4.3%至60.9%±1.3%。5种化疗药物对这两种细胞系的细胞毒作用均远低于rHTNF。评估了DDP或KSM与rHTNF联合使用时不同程度的协同增强细胞毒性。尤其在两种细胞系上，低剂量50kU/L的rHTNF与几乎所有浓度（10⁻⁴ - 10⁻¹ mg/L）的KSM均产生了明显的协同效应。
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