Allavena P, Bianchi G, Zhou D, van Damme J, Jílek P, Sozzani S, Mantovani A
Laboratory of Immunology, Istituto Mario Negri, Milano, Italy.
Eur J Immunol. 1994 Dec;24(12):3233-6. doi: 10.1002/eji.1830241249.
Under certain physiological and pathological conditions, natural killer (NK) cells rapidly accumulate in tissues. Chemokines are an essential component of the current paradigm of leukocyte recruitment. The present study was designed to investigate the responsiveness of NK cells to the prototypic C-C chemokine, monocyte chemotactic protein-1 (MCP-1). MCP-1 induced migration across filters of interleukin (IL)-2-activated NK cells, whereas it was a weak attractant for unstimulated cells. Maximal induction of migration required a positive concentration gradient between the lower and the upper compartment of the chemotaxis chamber. Preliminary characterization of the MCP-1 receptor on NK cells indicated that the chemotactic response to MCP-1 was blocked by pre-treatment of cells with Bordetella pertussis toxin, and MCP-1 but not IL-8 displaced 125I-labeled MCP-1 from IL-2-activated NK cells. The related chemokines MCP-2 and MCP-3 were also active--though less potent--attractants for activated NK cells. Thus the spectrum of action of MCP-1, -2 and -3 encompasses NK cells and chemokines are likely to play a role in regulating extravasation of these cells.
在某些生理和病理条件下，自然杀伤（NK）细胞会在组织中迅速积聚。趋化因子是当前白细胞募集模式的重要组成部分。本研究旨在调查NK细胞对典型C-C趋化因子单核细胞趋化蛋白-1（MCP-1）的反应性。MCP-1可诱导经白细胞介素（IL）-2激活的NK细胞穿过滤膜迁移，而对未受刺激的细胞而言，它是一种弱吸引剂。迁移的最大诱导需要趋化性小室上下腔室之间存在正浓度梯度。对NK细胞上MCP-1受体的初步表征表明，用百日咳博德特氏菌毒素预处理细胞可阻断对MCP-1的趋化反应，并且MCP-1而非IL-8可从经IL-2激活的NK细胞上置换出125I标记的MCP-1。相关趋化因子MCP-2和MCP-3对活化的NK细胞也是有活性的吸引剂，尽管效力较弱。因此，MCP-1、-2和-3的作用谱包括NK细胞，趋化因子可能在调节这些细胞的外渗中发挥作用。
