LLC-PK1肾上皮细胞中顶端心钠素受体的功能证据。

Functional evidence for an apical ANP receptor in LLC-PK1 kidney epithelial cells.

作者信息

Hashimoto Y, Ozaki J, Yasuhara M, Hori R, Suga S, Itoh H, Nakao K, Inui K

机构信息

Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Japan.

出版信息

Eur J Pharmacol. 1994 Aug 16;268(3):443-5. doi: 10.1016/0922-4106(94)90071-x.

DOI:10.1016/0922-4106(94)90071-x

PMID:7805770

Abstract

The localization of natriuretic peptide receptors was studied in LLC-PK1 cell monolayers grown on a micro-porous membrane filter. The addition of alpha-human atrial natriuretic peptide (alpha-hANP) to the apical side of the cells produced a concentration-dependent increase in cyclic GMP (cGMP) formation, the extent of which was similar to that produced by the addition to the basolateral side. More cGMP was formed with alpha-hANP (1 and 100 nM) than with the C-type natriuretic peptide (100 nM). These findings indicate that the biologically active receptors with high efficacy for alpha-hANP are expressed in both the apical and basolateral membranes in LLC-PK1 cells.

摘要

在生长于微孔膜滤器上的LLC - PK1细胞单层中研究了利钠肽受体的定位。将α - 人心房利钠肽（α - hANP）添加到细胞的顶端侧会使环磷酸鸟苷（cGMP）生成呈浓度依赖性增加，其程度与添加到基底外侧侧时相似。与C型利钠肽（100 nM）相比，α - hANP（1和100 nM）产生的cGMP更多。这些发现表明，对α - hANP具有高效能的生物活性受体在LLC - PK1细胞的顶端和基底外侧膜中均有表达。