Hayashi M, Hirai K, Unemoto T
Laboratory of Membrane Biochemistry, Faculty of Pharmaceutical Sciences, Chiba University, Japan.
FEBS Lett. 1995 Apr 17;363(1-2):75-7. doi: 10.1016/0014-5793(95)00283-f.
We previously cloned a part of nqr operon encoding the Na(+)-translocating NADH-quinone reductase (NQR) from the marine Vibrio alginolyticus [Hayashi et al., FEBS Lett. 356 (1994) 330-332]. From its nucleotide sequences, four consecutive open reading frames (ORF) encoding the gamma-subunit (27.7 kDa), two unidentified ORFs of 22.6 kDa and 21.5 kDa, and the beta-subunit (45.3 kDa) were recognized. The gene encoding the alpha-subunit was located upstream, and together with the recent report by Beattie et al. [FEBS Lett. 356 (1994) 333-338], the nqr operon was found to be constructed from six consecutive structural genes, where nqr1, nqr3 and nqr6 correspond to the alpha-, gamma-, and beta-subunits, respectively, of the NQR complex.
我们之前从海洋溶藻弧菌中克隆了编码Na⁺转运NADH-醌还原酶（NQR）的nqr操纵子的一部分[Hayashi等人，《欧洲生物化学学会联合会快报》356（1994）330 - 332]。从其核苷酸序列中，识别出了四个连续的开放阅读框（ORF），分别编码γ亚基（27.7 kDa）、两个未知的22.6 kDa和21.5 kDa的ORF以及β亚基（45.3 kDa）。编码α亚基的基因位于上游，并且与Beattie等人最近的报告[《欧洲生物化学学会联合会快报》356（1994）333 - 338]一起，发现nqr操纵子由六个连续的结构基因构成，其中nqr1、nqr3和nqr6分别对应于NQR复合物的α、γ和β亚基。
