Hoffmannová H, Novotná Z, Valouchová Z
SEVAC, Inc., Praha.
Folia Biol (Praha). 1994;40(3):125-33.
Cell lines established from African green monkey kidney cells have been used over years in virology. It is possible to distinguish them by detailed karyology of banded metaphases or by iso-enzyme studies. Different morphology of NOR-bearing chromosomes in three African green monkey cell lines studied in our laboratory enables us to distinguish them in simple conventionally Giemsa-stained slides. Vero cells have two chromosomes with secondary constrictions with active NORs. One chromosome is a small submetacentric with secondary constriction on the long arm in the vicinity of centromere. The second one is rather metacentric with a smaller secondary constriction. In BGM cells we found two submetacentric chromosomes, one has a large secondary constriction, the other has a smaller secondary constriction. BS-C-1 cells have a large metacentric chromosome with secondary constrictions on both arms. In one investigation an additional large acrocentric chromosome with the secondary constriction on the long arm was found. Comparison with morphology of NOR-bearing chromosomes in a Rhesus monkey cell line is discussed. The method described here is particularly useful for a rapid discrimination of cell lines, produced in our Institute, for virological and diagnostic application in virological laboratories of the hygiene service and in hospitals.
多年来，源自非洲绿猴肾细胞的细胞系一直被用于病毒学研究。可以通过带型中期染色体的详细核型分析或同工酶研究来区分它们。在我们实验室研究的三种非洲绿猴细胞系中，含核仁组织区（NOR）染色体的不同形态使我们能够在简单的常规吉姆萨染色玻片上区分它们。Vero细胞有两条带有活性NOR的具次缢痕染色体。一条染色体是小的亚中着丝粒染色体，在长臂靠近着丝粒处有次缢痕。第二条是相当近中着丝粒的，有较小的次缢痕。在BGM细胞中，我们发现两条亚中着丝粒染色体，一条有大的次缢痕，另一条有较小的次缢痕。BS - C - 1细胞有一条大的近中着丝粒染色体，两条臂上都有次缢痕。在一项研究中，还发现了一条额外的大近端着丝粒染色体，其长臂上有次缢痕。讨论了与恒河猴细胞系中含NOR染色体形态的比较。这里描述的方法对于快速鉴别我们研究所生产的用于卫生服务病毒学实验室和医院病毒学诊断应用的细胞系特别有用。
