Yulug I G, Egan S E, See C G, Fisher E M
Department of Biochemistry and Molecular Genetics, St. Mary's Hospital Medical School, Imperial College, London, United Kingdom.
Genomics. 1994 Jul 15;22(2):313-8. doi: 10.1006/geno.1994.1389.
We have mapped GRB2, a signal transduction gene whose protein product is an essential component of the pathway between tyrosine kinases (such as the epidermal growth factor receptor) and downstream proteins (such as Ras and Sos). We assigned GRB2 to human chromosome 17 by hybridization to a somatic cell hybrid mapping panel. To position the locus at a much finer resolution, we have isolated the human GRB2 gene in three different cosmids, which we have mapped by fluorescence in situ hybridization to the long arm of human chromosome 17 (17q24-q25). We have hybridized a human GRB2 open reading frame probe to mouse DNAs from the European Interspecific Backcross. The segregation patterns reveal that the mouse Grb2 locus maps distally on chromosome 11, and an additional Grb2-related locus is present on chromosome 4 of one of the parental strains, Mus spretus/CRC.
我们已经绘制出GRB2的图谱，GRB2是一种信号转导基因，其蛋白质产物是酪氨酸激酶（如表皮生长因子受体）与下游蛋白质（如Ras和Sos）之间信号通路的重要组成部分。我们通过与体细胞杂交定位板杂交，将GRB2定位于人类17号染色体。为了以更高的分辨率定位该基因座，我们在三种不同的黏粒中分离出人类GRB2基因，并通过荧光原位杂交将其定位于人类17号染色体长臂（17q24 - q25）。我们用人类GRB2开放阅读框探针与欧洲种间回交小鼠的DNA进行杂交。分离模式显示，小鼠Grb2基因座定位于11号染色体远端，并且在亲本菌株之一的小家鼠/CRC的4号染色体上存在一个额外的与Grb2相关的基因座。
