Kumar A, Malaviya A N, Srivastava L M
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi.
Immunobiology. 1994 Aug;191(1):9-20. doi: 10.1016/S0171-2985(11)80264-0.
The number of C3b receptors (CR1) on erythrocytes (E) has been quantitated by whole cell enzyme-linked immunosorbent assay (ELISA), using a monoclonal anti-CR1 antibody, in 46 healthy individuals as controls, 58 having rheumatoid arthritis (RA) and 3 hereditary angioedema patients. The mean value of CR1 in RA (381/E) was significantly lower (p < 0.001) when compared to normal controls (646/E). In hereditary angioedema patients CR1 numbers (620/E) were found to be comparable to normal values. No significant difference was found between normal male (708/E) and female (598/E) subjects. Among the patient groups, those on steroid therapy (352/E) showed no change compared to others not receiving such therapy (408/E). The cumulative frequency curve of CR1 in the normal population showed maximum inflection at 32% and 82%. This led us to conclude that there is a trimodal distribution of receptors in the control population. Such a contention is well supported by frequency histogram, when a small group interval (0-50) was chosen. However, if large group intervals (0-100 or 0-150) were considered, a very close approximation to unimodal pattern was obtained. The factors actually contributing to low receptor numbers are yet to be elucidated.
采用单克隆抗C3b受体（CR1）抗体，通过全细胞酶联免疫吸附测定（ELISA）对46名健康对照者、58名类风湿性关节炎（RA）患者和3名遗传性血管性水肿患者红细胞（E）上的CR1数量进行了定量分析。与正常对照组（646/E）相比，RA患者的CR1平均值（381/E）显著降低（p < 0.001）。在遗传性血管性水肿患者中，CR1数量（620/E）与正常值相当。正常男性（708/E）和女性（598/E）受试者之间未发现显著差异。在患者组中，接受类固醇治疗的患者（352/E）与未接受此类治疗的患者（408/E）相比无变化。正常人群中CR1的累积频率曲线在32%和82%处出现最大拐点。这使我们得出结论，对照组人群中受体存在三峰分布。当选择较小的组距（0 - 50）时，频率直方图很好地支持了这一论点。然而，如果考虑较大的组距（0 - 100或0 - 150），则可得到非常接近单峰模式的结果。导致受体数量降低的实际因素尚待阐明。
