Patel S, Dumsha T C, Sydiskis R J
Department of Endodontics, University of Maryland, Baltimore, College of Dental Surgery, MD 21201.
Int Endod J. 1994 Jan;27(1):1-5. doi: 10.1111/j.1365-2591.1994.tb00220.x.
One of the key factors for obtaining a favourable long-term prognosis in avulsed teeth is maintenance of the vitality of the periodontal ligament (PDL) cells. Most studies which have examined PDL cell vitality have used neutral red or trypan blue as stains. However, these stains have certain inherent disadvantages. The purpose of this paper was to (i) evaluate the use of saline and milk as storage media for PDL cells and (ii) determine the value of using fluorescein diacetate (FDA) as a staining medium for vital PDL cells on the root surface of avulsed teeth. Thirty-two single-rooted premolars were utilized from patients aged 13 to 28 years. Following atraumatic extraction, the teeth in the experimental groups were air dried for 10 min and then placed in either milk or saline for 120 min. Both control and experimental teeth were subjected to trypsinization procedures, staining with FDA, and haemocytometer readings to determine the number of vital cells. There was no statistically significant difference in the number of viable cells on the root surfaces of teeth after 2 h of storage in either milk or in saline. Furthermore, staining with FDA provided an excellent method by which to determine PDL cell vitality.
获得脱位牙良好长期预后的关键因素之一是维持牙周膜（PDL）细胞的活力。大多数研究PDL细胞活力的实验都使用中性红或台盼蓝作为染色剂。然而，这些染色剂有一些固有的缺点。本文的目的是：（i）评估使用生理盐水和牛奶作为PDL细胞的储存介质；（ii）确定使用荧光素二乙酸酯（FDA）作为脱位牙根表面存活PDL细胞的染色介质的价值。从13至28岁的患者中选取了32颗单根前磨牙。在无创拔牙后，将实验组的牙齿风干10分钟，然后置于牛奶或生理盐水中120分钟。对照牙和实验牙都经过胰蛋白酶处理、用FDA染色，并通过血细胞计数器读数来确定活细胞的数量。在牛奶或生理盐水中储存2小时后，牙根表面活细胞的数量没有统计学上的显著差异。此外，用FDA染色提供了一种很好的确定PDL细胞活力的方法。
