Doyle D L, Dumsha T C, Sydiskis R J
Dept. of Endodontics, Baltimore College of Dental Surgery, University of Maryland at Baltimore 21201, USA.
Endod Dent Traumatol. 1998 Oct;14(5):221-4. doi: 10.1111/j.1600-9657.1998.tb00843.x.
This study was designed to evaluate the effect of soaking in either Hank's balanced salt solution (HBSS) or milk on periodontal ligament (PDL) cell viability in avulsed teeth. Dry storage times of 30, 60, and 90 min were evaluated. PDL cell viability was determined after removal of the cells from the root surfaces of extracted teeth using a modification of the procedure described by Nakashima (Arch Oral Biol 1991;36:655-63). After trypsinization and subsequent treatment in collagenase, the cells were stained with trypan blue, and viable and non-viable cells were counted using a hemocytometer and converted to percentages for statistical comparison. The results of this study demonstrated no significant difference in the number of viable cells with or without soaking in HBSS or milk at any of the dry storage times. In addition, there was no significant difference in PDL cell viability between the 30-and the 60-min dry periods. Although the soaking procedure had no obvious negative consequence, no significant improvement in PDL cell viability by the addition of this step was demonstrated under the conditions of this study.
本研究旨在评估将脱位牙浸泡于汉克斯平衡盐溶液（HBSS）或牛奶中对牙周膜（PDL）细胞活力的影响。评估了30分钟、60分钟和90分钟的干燥保存时间。使用中岛描述方法（《口腔生物学文献》1991年；36:655 - 63）的改良方法从拔除牙齿的牙根表面分离细胞后，测定PDL细胞活力。经胰蛋白酶消化并随后用胶原酶处理后，细胞用台盼蓝染色，使用血细胞计数器对活细胞和死细胞进行计数，并换算成百分比进行统计学比较。本研究结果表明，在任何干燥保存时间下，浸泡于HBSS或牛奶与否，活细胞数量均无显著差异。此外，30分钟和60分钟干燥保存期之间，PDL细胞活力也无显著差异。尽管浸泡步骤没有明显的负面作用，但在本研究条件下，未证明添加该步骤能显著提高PDL细胞活力。
