E-钙黏蛋白和腺瘤性息肉病蛋白（APC）竞争与β-连环蛋白及细胞骨架的相互作用。
E-cadherin and APC compete for the interaction with beta-catenin and the cytoskeleton.
作者信息
Hülsken J, Birchmeier W, Behrens J
机构信息
Max-Delbrück-Center for Molecular Medicine, Berlin, Germany.
出版信息
J Cell Biol. 1994 Dec;127(6 Pt 2):2061-9. doi: 10.1083/jcb.127.6.2061.
Abstract
beta-Catenin is involved in the formation of adherens junctions of mammalian epithelia. It interacts with the cell adhesion molecule E-cadherin and also with the tumor suppressor gene product APC, and the Drosophila homologue of beta-catenin, armadillo, mediates morphogenetic signals. We demonstrate here that E-cadherin and APC directly compete for binding to the internal, armadillo-like repeats of beta-catenin; the NH2-terminal domain of beta-catenin mediates the interaction of the alternative E-cadherin and APC complexes to the cytoskeleton by binding to alpha-catenin. Plakoglobin (gamma-catenin), which is structurally related to beta-catenin, mediates identical interactions. We thus show that the APC tumor suppressor gene product forms strikingly similar associations as found in cell junctions and suggest that beta-catenin and plakoglobin are central regulators of cell adhesion, cytoskeletal interaction, and tumor suppression.
摘要
β-连环蛋白参与哺乳动物上皮细胞黏附连接的形成。它与细胞黏附分子E-钙黏蛋白相互作用，也与肿瘤抑制基因产物APC相互作用，并且β-连环蛋白在果蝇中的同源物犰狳介导形态发生信号。我们在此证明，E-钙黏蛋白和APC直接竞争与β-连环蛋白内部的犰狳样重复序列结合；β-连环蛋白的NH2末端结构域通过与α-连环蛋白结合，介导替代的E-钙黏蛋白和APC复合物与细胞骨架的相互作用。与β-连环蛋白结构相关的桥粒芯蛋白（γ-连环蛋白）介导相同的相互作用。因此，我们表明APC肿瘤抑制基因产物形成了与细胞连接中发现的惊人相似的关联，并表明β-连环蛋白和桥粒芯蛋白是细胞黏附、细胞骨架相互作用和肿瘤抑制的核心调节因子。
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E-cadherin and APC compete for the interaction with beta-catenin and the cytoskeleton.E-钙黏蛋白和腺瘤性息肉病蛋白（APC）竞争与β-连环蛋白及细胞骨架的相互作用。
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2
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A novel moesin-, ezrin-, radixin-like gene is a candidate for the neurofibromatosis 2 tumor suppressor.一种新的类肌动蛋白结合蛋白、埃兹蛋白、根蛋白样基因是2型神经纤维瘤病肿瘤抑制基因的候选基因。
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