Cantatore P, Roberti M, Pesole G, Ludovico A, Milella F, Gadaleta M N, Saccone C
Department of Biochemistry and Molecular Biology, University of Bari, Italy.
J Mol Evol. 1994 Dec;39(6):589-97. doi: 10.1007/BF00160404.
To obtain information relative to the phylogenesis and microevolutionary rate of fish mitochondrial DNA, the nucleotide sequence of cytochrome b gene in seven fish species belonging to the order of Perciformes was determined. Sequence analysis showed that fish mitochondrial DNA has a nucleotide compositional bias similar to that of sharks but lower compared to mammals and birds. Quantitative evolutionary analysis, carried out by using a markovian stochastic model, clarifies some phylogenetic relationships within the Perciformes order, particularly in the Scombridae family, and between Perciformes, Gadiformes, Cypriniformes, and Acipenseriformes. The molecular clock of mitochondrial DNA was calibrated with the nucleotide substitution rate of cytochrome b gene in five shark species having divergence times inferred from paleontological estimates. The results of such analysis showed that Acipenseriformes diverged from Perciformes by about 200 MY, that the Perciformes common ancestor dates back to 150 MY, and that fish mitochondrial DNA has a nucleotide substitution rate three to five times lower than that of mammals.
为获取有关鱼类线粒体DNA系统发育和微观进化速率的信息，测定了鲈形目七种鱼类细胞色素b基因的核苷酸序列。序列分析表明，鱼类线粒体DNA的核苷酸组成偏向与鲨鱼相似，但与哺乳动物和鸟类相比更低。通过使用马尔可夫随机模型进行的定量进化分析，阐明了鲈形目内部的一些系统发育关系，特别是在鲭科中，以及鲈形目、鳕形目、鲤形目和鲟形目之间的关系。利用从古生物学估计推断出分歧时间的五种鲨鱼物种细胞色素b基因的核苷酸替代率，对线粒体DNA的分子钟进行了校准。该分析结果表明，鲟形目与鲈形目大约在2亿年前分化，鲈形目的共同祖先可追溯到1.5亿年前，并且鱼类线粒体DNA的核苷酸替代率比哺乳动物低三到五倍。
