[通过聚合酶链反应检测经支气管活检标本中的结核分枝杆菌]

[Detection of Mycobacterium tuberculosis in transbronchial biopsy specimens by polymerase chain reaction].

作者信息

Yamada M, Furuse K, Kawahara M, Ogawara M, Atagi S, Okada T, Kawaguchi Y, Kamimori T, Nakao M, Ueda E

机构信息

Department of Internal Medicine, National Kinki Central Hospital, Osaka, Japan.

出版信息

Nihon Kyobu Shikkan Gakkai Zasshi. 1994 Aug;32(8):747-51.

PMID:7807753

Abstract

Polymerase chain reaction (PCR) for the rapid detection of Mycobacterium tuberculosis was used to test 30 clinical specimens (transbronchial biopsy specimens) from patients in whom tuberculosis was suspected on chest X-ray. In these patients, 15 cases were histologically diagnosed as tuberculosis. Among them, M. tuberculosis was detected in 8 specimens by PCR. Of 8 PCR positive specimens, 7 were also positive by smear and culture; the other was negative by smear and positive by culture. On the other hand, 15 cases were therapeutically diagnosed as the tuberculosis and these specimens showed negative results in neither PCR nor microbiological tests. We conclude that the PCR method is useful for rapid and direct detection of M. tuberculosis in transbronchial biopsy specimens.

摘要

采用聚合酶链反应（PCR）快速检测结核分枝杆菌，对30例胸部X线怀疑患有结核病患者的临床标本（经支气管活检标本）进行检测。在这些患者中，15例经组织学诊断为结核病。其中，通过PCR在8份标本中检测到结核分枝杆菌。在8份PCR阳性标本中，7份涂片和培养也呈阳性；另1份涂片阴性但培养阳性。另一方面，15例经治疗诊断为结核病，这些标本的PCR和微生物检测均为阴性。我们得出结论，PCR方法可用于经支气管活检标本中结核分枝杆菌的快速直接检测。