Kolot M, Yagil E
Department of Biochemistry, Tel-Aviv University, Israel.
Mol Gen Genet. 1994 Dec 1;245(5):623-7. doi: 10.1007/BF00282225.
The positions of the endonucleolytic cleavages promoted by the integrase protein (Int) of coliphage HK022 within its attB site were determined. The protein catalyses a staggered cut, which defines an overlap sequence of 7 bp within the core site. The overlap region is at the center of symmetry of a palindromic sequence which appears in all four putative att core binding sites for Int. We confirm that the order of strand exchange is similar to that in phage lambda.
确定了大肠杆菌噬菌体HK022整合酶蛋白（Int）在其attB位点内促进的核酸内切酶切割位置。该蛋白催化交错切割，这在核心位点内定义了一个7个碱基对的重叠序列。重叠区域位于回文序列的对称中心，该回文序列出现在Int的所有四个假定att核心结合位点中。我们证实链交换的顺序与噬菌体λ中的相似。
