恶性疟原虫类质体样DNA的rpoB基因的系统发育分析。

Phylogenetic analysis of the rpoB gene from the plastid-like DNA of Plasmodium falciparum.

作者信息

Gardner M J, Goldman N, Barnett P, Moore P W, Rangachari K, Strath M, Whyte A, Williamson D H, Wilson R J

机构信息

Parasitology Division, National Institute for Medical Research, Mill Hill, London, UK.

出版信息

Mol Biochem Parasitol. 1994 Aug;66(2):221-31. doi: 10.1016/0166-6851(94)90149-x.

DOI:10.1016/0166-6851(94)90149-x

PMID:7808472

Abstract

Malaria and other Apicomplexan parasites harbour two extrachromosomal DNAs. One is mitochondrial and the other is a 35-kb circle with some plastid-like features but whose provenance and function is unknown. In addition to genes for rRNAs, tRNAs and ribosomal proteins, the 35-kb circular DNA of Plasmodium falciparum carries an rpoBC operon which encodes subunits of a eubacteria-like RNA polymerase. The phylogenetic analysis of the complete rpoB sequence presented here supports our inference that the 35-kb circle is the remnant of a plastid genome.

摘要

疟疾和其他顶复门寄生虫含有两种染色体外DNA。一种是线粒体DNA，另一种是35千碱基对的环状DNA，具有一些类似质体的特征，但其来源和功能尚不清楚。除了rRNA、tRNA和核糖体蛋白的基因外，恶性疟原虫的35千碱基对环状DNA还携带一个rpoBC操纵子，该操纵子编码一种类似真细菌的RNA聚合酶的亚基。此处给出的完整rpoB序列的系统发育分析支持了我们的推断，即35千碱基对的环状DNA是质体基因组的残余物。