疟原虫中染色体外DNA的母系遗传。

Maternal inheritance of extrachromosomal DNA in malaria parasites.

作者信息

Creasey A, Mendis K, Carlton J, Williamson D, Wilson I, Carter R

机构信息

Division of Biological Sciences, I.C.A.P.B., University of Edinburgh, UK.

出版信息

Mol Biochem Parasitol. 1994 May;65(1):95-8. doi: 10.1016/0166-6851(94)90118-x.

DOI:10.1016/0166-6851(94)90118-x

PMID:7935632

Abstract

Plasmodium falciparum has two extrachromosomal genomes, the mitochondrial 6-kb DNA element and the 35-kb circular DNA. The mitochondrial gene cytochrome b on the 6-kb element has been shown to be inherited uniparentally. In order to ascertain whether the route is maternal or paternal we have examined preparations of male and female gametes of the closely related Plasmodium gallinaceum for the presence of extrachromosomal DNA. DNA from purified preparations of gametes was hybridised to probes for both the 6-kb and 35-kb extrachromosomal genomes. Both probes hybridised to the preparation of Plasmodium gallinaceum female gametes but not to that of the males. We conclude that the extrachromosomal DNAs of malaria parasites are transmitted maternally.

摘要

恶性疟原虫有两个染色体外基因组，即线粒体6千碱基对的DNA元件和35千碱基对的环状DNA。已证明6千碱基对元件上的线粒体基因细胞色素b是单亲遗传的。为了确定这条遗传途径是母系遗传还是父系遗传，我们检查了亲缘关系密切的鸡疟原虫的雄配子和雌配子制剂中是否存在染色体外DNA。来自纯化配子制剂的DNA与针对6千碱基对和35千碱基对染色体外基因组的探针进行杂交。两种探针都与鸡疟原虫雌配子的制剂杂交，但不与雄配子的制剂杂交。我们得出结论，疟原虫的染色体外DNA是通过母系遗传的。