Agarwal S, Suzuki J B, Piesco N P, Aichelmann-Reidy M B
Division of Oral Biology, University of Pittsburgh, Pennsylvania.
Oral Microbiol Immunol. 1994 Oct;9(5):262-71. doi: 10.1111/j.1399-302x.1994.tb00069.x.
Polymorphonuclear leukocytes (neutrophils) in patients with localized juvenile periodontitis with decreased chemotaxis exhibit increased adherence. The molecular basis of increased adherence of neutrophils in localized juvenile periodontitis is not clear. We show that the neutrophils from localized juvenile periodontitis patients with decreased neutrophil chemotaxis and increased adherence exhibit increased expression of the CD11/CD18 family of adherence molecules, Mac-1, leukocyte function-associated antigen (LFA-1) and p150,95, as compared with neutrophils obtained from systemically and periodontally healthy controls. Treatment of neutrophils obtained from healthy subjects with sera from localized juvenile periodontitis patients resulted in an increased expression of Mac-1, leukocyte function-associated antigen and p150,95 molecules. Sera obtained from health subjects, patients with adult periodontitis or localized juvenile periodontitis patients with normal chemotaxis did not increase the expression of CD11/CD18 molecules on the neutrophils obtained from healthy subjects. The ability of localized juvenile periodontitis sera to induce expression of adherence molecules was at least partially inhibited by pretreatment of localized juvenile periodontitis sera with anti-tumor necrosis factor and anti-interleukin-1 antibodies. Furthermore, increasing concentrations of rh-tumor necrosis factor-alpha and interleukin-1 beta also induced increased expression of the Mac-1 molecule on neutrophils from localized juvenile periodontitis patients and healthy subjects. The increased expression of adherence molecules on neutrophils from localized juvenile periodontitis patients was sustained and could not be reversed by treatment of localized juvenile periodontitis neutrophils with sera obtained from healthy subjects. The upregulation of adherence molecules on neutrophils by localized juvenile periodontitis sera in these experiments emphasizes the physiologic importance of quantitatively small but biologically significant levels of cytokines in the modulation of neutrophil functions in localized juvenile periodontitis.
局部性青少年牙周炎患者中趋化性降低的多形核白细胞（中性粒细胞）表现出黏附性增加。局部性青少年牙周炎中中性粒细胞黏附性增加的分子基础尚不清楚。我们发现，与从全身和牙周健康的对照者获取的中性粒细胞相比，来自局部性青少年牙周炎患者且趋化性降低和黏附性增加的中性粒细胞，其黏附分子CD11/CD18家族、Mac-1、白细胞功能相关抗原（LFA-1）和p150,95的表达增加。用局部性青少年牙周炎患者的血清处理从健康受试者获取的中性粒细胞，导致Mac-1、白细胞功能相关抗原和p150,95分子的表达增加。从健康受试者、成人牙周炎患者或趋化性正常的局部性青少年牙周炎患者获取的血清，并未增加从健康受试者获取的中性粒细胞上CD11/CD18分子的表达。局部性青少年牙周炎血清诱导黏附分子表达的能力，至少部分被用抗肿瘤坏死因子和抗白细胞介素-1抗体预处理局部性青少年牙周炎血清所抑制。此外，重组人肿瘤坏死因子-α和白细胞介素-1β浓度的增加，也诱导了局部性青少年牙周炎患者和健康受试者中性粒细胞上Mac-1分子的表达增加。局部性青少年牙周炎患者中性粒细胞上黏附分子表达的增加是持续的，用健康受试者的血清处理局部性青少年牙周炎中性粒细胞并不能使其逆转。在这些实验中，局部性青少年牙周炎血清对中性粒细胞黏附分子的上调强调了在局部性青少年牙周炎中，数量少但生物学意义重大的细胞因子水平在调节中性粒细胞功能方面的生理重要性。
