Yamaguchi N, Kawasaki M, Yamashita Y, Nakashima K, Koga T
Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka, Japan.
Infect Immun. 1995 Dec;63(12):4589-94. doi: 10.1128/iai.63.12.4589-4594.1995.
Serotype b-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans Y4 consists of D-fucose and L-rhamnose. To clarify the role of SPA in phagocytosis of the organism by human polymorphonuclear leukocytes (PMNs), monoclonal antibodies (MAbs) against SPA and SPA-defective mutants, which were constructed by inserting the transposon Tn916 into strain Y4, were used in a chemiluminescence (CL) assay and a phagocytic killing assay. The CL responses of human PMNs to strain Y4 were very low, and the organism was not killed by PMNs. In contrast, SPA-defective mutants induced strong CL responses. The addition of immunoglobulin G MAbs against Y4 SPA enhanced significantly both the CL responses to strain Y4 and the killing of the organism in the presence of complement. The CL responses to SPA-defective mutants were little affected by the addition of these MAbs. We conclude that SPA of A. actinomycetemcomitans plays an important role in the resistance to host defenses by PMNs.
伴放线放线杆菌Y4的b型特异性多糖抗原（SPA）由D-岩藻糖和L-鼠李糖组成。为了阐明SPA在人多形核白细胞（PMN）对该菌的吞噬作用中的作用，在化学发光（CL）试验和吞噬杀伤试验中使用了针对SPA的单克隆抗体（MAb）以及通过将转座子Tn916插入Y4菌株构建的SPA缺陷型突变体。人PMN对Y4菌株的CL反应非常低，并且该菌未被PMN杀死。相反，SPA缺陷型突变体诱导强烈的CL反应。添加针对Y4 SPA的免疫球蛋白G MAb在补体存在下显著增强了对Y4菌株的CL反应以及对该菌的杀伤作用。添加这些MAb对SPA缺陷型突变体的CL反应影响很小。我们得出结论，伴放线放线杆菌的SPA在PMN对宿主防御的抵抗中起重要作用。
