类荚膜多糖血清型特异性抗原在伴放线放线杆菌抵抗人多形核白细胞吞噬作用中的作用。
Role of the capsular polysaccharide-like serotype-specific antigen in resistance of Actinobacillus actinomycetemcomitans to phagocytosis by human polymorphonuclear leukocytes.
作者信息
Yamaguchi N, Kawasaki M, Yamashita Y, Nakashima K, Koga T
机构信息
Department of Preventive Dentistry, Kyushu University Faculty of Dentistry, Fukuoka, Japan.
出版信息
Infect Immun. 1995 Dec;63(12):4589-94. doi: 10.1128/iai.63.12.4589-4594.1995.
Abstract
Serotype b-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans Y4 consists of D-fucose and L-rhamnose. To clarify the role of SPA in phagocytosis of the organism by human polymorphonuclear leukocytes (PMNs), monoclonal antibodies (MAbs) against SPA and SPA-defective mutants, which were constructed by inserting the transposon Tn916 into strain Y4, were used in a chemiluminescence (CL) assay and a phagocytic killing assay. The CL responses of human PMNs to strain Y4 were very low, and the organism was not killed by PMNs. In contrast, SPA-defective mutants induced strong CL responses. The addition of immunoglobulin G MAbs against Y4 SPA enhanced significantly both the CL responses to strain Y4 and the killing of the organism in the presence of complement. The CL responses to SPA-defective mutants were little affected by the addition of these MAbs. We conclude that SPA of A. actinomycetemcomitans plays an important role in the resistance to host defenses by PMNs.
摘要
伴放线放线杆菌Y4的b型特异性多糖抗原（SPA）由D-岩藻糖和L-鼠李糖组成。为了阐明SPA在人多形核白细胞（PMN）对该菌的吞噬作用中的作用，在化学发光（CL）试验和吞噬杀伤试验中使用了针对SPA的单克隆抗体（MAb）以及通过将转座子Tn916插入Y4菌株构建的SPA缺陷型突变体。人PMN对Y4菌株的CL反应非常低，并且该菌未被PMN杀死。相反，SPA缺陷型突变体诱导强烈的CL反应。添加针对Y4 SPA的免疫球蛋白G MAb在补体存在下显著增强了对Y4菌株的CL反应以及对该菌的杀伤作用。添加这些MAb对SPA缺陷型突变体的CL反应影响很小。我们得出结论，伴放线放线杆菌的SPA在PMN对宿主防御的抵抗中起重要作用。
相似文献
1
Role of the capsular polysaccharide-like serotype-specific antigen in resistance of Actinobacillus actinomycetemcomitans to phagocytosis by human polymorphonuclear leukocytes.类荚膜多糖血清型特异性抗原在伴放线放线杆菌抵抗人多形核白细胞吞噬作用中的作用。
Infect Immun. 1995 Dec;63(12):4589-94. doi: 10.1128/iai.63.12.4589-4594.1995.
2
Construction of mutants of Actinobacillus actinomycetemcomitans defective in serotype b-specific polysaccharide antigen by insertion of transposon Tn916.通过转座子Tn916插入构建血清型b特异性多糖抗原缺陷的伴放线放线杆菌突变体。
J Gen Microbiol. 1992 Jun;138(6):1203-9. doi: 10.1099/00221287-138-6-1203.
3
Binding of the capsule-like serotype-specific polysaccharide antigen and the lipopolysaccharide from Actinobacillus actinomycetemcomitans to human complement-derived opsonins.伴放线放线杆菌的荚膜样血清型特异性多糖抗原和脂多糖与人补体衍生调理素的结合。
Oral Microbiol Immunol. 1998 Dec;13(6):348-54. doi: 10.1111/j.1399-302x.1998.tb00690.x.
4
Immunogenicity of Actinobacillus actinomycetemcomitans serotype b-specific polysaccharide-protein conjugate.
Oral Microbiol Immunol. 1996 Aug;11(4):220-5. doi: 10.1111/j.1399-302x.1996.tb00173.x.
5
Identification of a genetic locus essential for serotype b-specific antigen synthesis in Actinobacillus actinomycetemcomitans.在伴放线放线杆菌中鉴定b型特异性抗原合成所必需的基因座。
Infect Immun. 1998 Jan;66(1):107-14. doi: 10.1128/IAI.66.1.107-114.1998.
6
Role of serotype-specific polysaccharide in the resistance of Streptococcus mutans to phagocytosis by human polymorphonuclear leukocytes.血清型特异性多糖在变形链球菌抵抗人多形核白细胞吞噬作用中的作用。
Infect Immun. 2000 Feb;68(2):644-50. doi: 10.1128/IAI.68.2.644-650.2000.
7
Role of prostaglandin in the formation of osteoclasts induced by capsular-like polysaccharide antigen of Actinobacillus actinomycetemcomitans strain Y4.前列腺素在伴放线放线杆菌Y4菌株荚膜样多糖抗原诱导破骨细胞形成中的作用
Oral Microbiol Immunol. 1995 Apr;10(2):69-75. doi: 10.1111/j.1399-302x.1995.tb00121.x.
8
Extracellular 37-kDa antigenic protein from Actinobacillus actinomycetemcomitans induces TNF-alpha, IL-1 beta, and IL-6 in murine macrophages.伴放线放线杆菌的细胞外37 kDa抗原蛋白可在小鼠巨噬细胞中诱导肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6的产生。
J Dent Res. 1997 Sep;76(9):1538-47. doi: 10.1177/00220345970760090501.
9
Differentiation of the serotype b and species-specific antigens of Actinobacillus actinobacillus actinomycetemcomitans recognized by monoclonal antibodies.通过单克隆抗体识别的伴放线放线杆菌b血清型和种特异性抗原的鉴别
Oral Microbiol Immunol. 1996 Aug;11(4):209-19. doi: 10.1111/j.1399-302x.1996.tb00172.x.
10
Opsonic antibody activity against Actinobacillus actinomycetemcomitans in patients with rapidly progressive periodontitis.快速进展性牙周炎患者针对伴放线放线杆菌的调理素抗体活性
Infect Immun. 1992 Nov;60(11):4819-25. doi: 10.1128/iai.60.11.4819-4825.1992.
引用本文的文献
1
Increased sensitivity of Aggregatibacter actinomycetemcomitans to human serum is mediated by induction of a bacteriophage.牙龈卟啉单胞菌对人血清敏感性增加是由噬菌体诱导介导的。
Mol Oral Microbiol. 2023 Feb;38(1):58-70. doi: 10.1111/omi.12378. Epub 2022 Jul 25.
2
O-Polysaccharide Plays a Major Role on the Virulence and Immunostimulatory Potential of During Periodontal Infection.O-多糖在牙周感染期间对 的毒力和免疫刺激潜力起着重要作用。
Front Immunol. 2020 Oct 30;11:591240. doi: 10.3389/fimmu.2020.591240. eCollection 2020.
3
Differential expression of CC chemokines (CCLs) and receptors (CCRs) by human T lymphocytes in response to different Aggregatibacter actinomycetemcomitans serotypes.人T淋巴细胞对不同血清型伴放线聚集杆菌的应答中CC趋化因子（CCL）及其受体（CCR）的差异表达
J Appl Oral Sci. 2015 Nov-Dec;23(6):580-90. doi: 10.1590/1678-775720150285.
4
Differential expression of CC chemokines (CCLs) and receptors (CCRs) by human T lymphocytes in response to different Aggregatibacter actinomycetemcomitans serotypes.人T淋巴细胞对不同血清型伴放线聚集杆菌反应时CC趋化因子（CCLs）和受体（CCRs）的差异表达
J Appl Oral Sci. 2015 Oct;23(5):536-46. doi: 10.1590/1678-775720150285.
5
Azithromycin enhances phagocytic killing of Aggregatibacter actinomycetemcomitans Y4 by human neutrophils.阿奇霉素增强人中性粒细胞对伴放线聚集杆菌Y4的吞噬杀伤作用。
J Periodontol. 2015 Jan;86(1):155-61. doi: 10.1902/jop.2014.140183.
6
LL-37 opsonizes and inhibits biofilm formation of Aggregatibacter actinomycetemcomitans at subbactericidal concentrations.LL-37 在亚抑菌浓度下调理并抑制伴放线放线杆菌生物膜的形成。
Infect Immun. 2013 Oct;81(10):3577-85. doi: 10.1128/IAI.01288-12. Epub 2013 Jul 8.
7
Both leukotoxin and poly-N-acetylglucosamine surface polysaccharide protect Aggregatibacter actinomycetemcomitans cells from macrophage killing.白细胞毒素和聚-N-乙酰葡糖胺表面多糖均可保护伴放线聚集杆菌细胞免受巨噬细胞杀伤。
Microb Pathog. 2008 Sep;45(3):173-80. doi: 10.1016/j.micpath.2008.05.007. Epub 2008 Jun 5.
8
Actinobacillus actinomycetemcomitans serotype d-specific antigen contains the O antigen of lipopolysaccharide.伴放线放线杆菌血清型d特异性抗原包含脂多糖的O抗原。
Infect Immun. 2003 Sep;71(9):5005-11. doi: 10.1128/IAI.71.9.5005-5011.2003.
9
Effect of ciprofloxacin on killing of Actinobacillus actinomycetemcomitans by polymorphonuclear leukocytes.环丙沙星对多形核白细胞杀灭伴放线放线杆菌的影响。
Antimicrob Agents Chemother. 2002 Jun;46(6):1980-4. doi: 10.1128/AAC.46.6.1980-1984.2002.
10
Identification of Actinobacillus actinomycetemcomitans serotypes by multiplex PCR.通过多重聚合酶链反应鉴定伴放线放线杆菌血清型
J Clin Microbiol. 2001 May;39(5):2002-5. doi: 10.1128/JCM.39.5.2002-2005.2001.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The role of the host response in periodontal disease progression: implications for future treatment strategies.宿主反应在牙周病进展中的作用：对未来治疗策略的启示。
J Periodontol. 1993 Aug;64(8 Suppl):792-806. doi: 10.1902/jop.1993.64.8s.792.
3
Seroclassification of hitherto nontypeable Actinobacillus actinomycetemcomitans strains: evidence for a new serotype e.迄今不可分型的伴放线放线杆菌菌株的血清分型：新型血清型e的证据
Oral Microbiol Immunol. 1993 Apr;8(2):116-20. doi: 10.1111/j.1399-302x.1993.tb00556.x.
4
Conjugate vaccines: practice and theory.结合疫苗：实践与理论
Springer Semin Immunopathol. 1993;15(2-3):217-26. doi: 10.1007/BF00201102.
5
Serum antibody opsonic activity against Actinobacillus actinomycetemcomitans in human periodontal diseases.人类牙周疾病中针对伴放线放线杆菌的血清抗体调理活性。
J Infect Dis. 1993 Dec;168(6):1436-43. doi: 10.1093/infdis/168.6.1436.
6
Role of YadA in resistance of Yersinia enterocolitica to phagocytosis by human polymorphonuclear leukocytes.耶尔森氏菌粘附素A（YadA）在小肠结肠炎耶尔森菌抵抗人多形核白细胞吞噬作用中的作用
Infect Immun. 1994 Apr;62(4):1275-81. doi: 10.1128/iai.62.4.1275-1281.1994.
7
Immunoglobulin G2 antibodies promote neutrophil killing of Actinobacillus actinomycetemcomitans.免疫球蛋白G2抗体促进中性粒细胞对伴放线放线杆菌的杀伤作用。
Infect Immun. 1995 Mar;63(3):1070-5. doi: 10.1128/iai.63.3.1070-1075.1995.
8
Resistance of a Tn4351-generated polysaccharide mutant of Porphyromonas gingivalis to polymorphonuclear leukocyte killing.牙龈卟啉单胞菌Tn4351诱导的多糖突变体对多形核白细胞杀伤的抗性
Infect Immun. 1995 Feb;63(2):393-401. doi: 10.1128/iai.63.2.393-401.1995.
9
Neutrophil function in juvenile periodontitis: induction of adherence.青少年牙周炎中的中性粒细胞功能：黏附诱导
Oral Microbiol Immunol. 1994 Oct;9(5):262-71. doi: 10.1111/j.1399-302x.1994.tb00069.x.
10
Human immunoglobulin G (IgG) Fc receptor IIA (CD32) polymorphism and IgG2-mediated bacterial phagocytosis by neutrophils.人类免疫球蛋白G（IgG）Fc受体IIA（CD32）多态性与中性粒细胞介导的IgG2细菌吞噬作用
Infect Immun. 1995 Jan;63(1):73-81. doi: 10.1128/iai.63.1.73-81.1995.
Zotero 插件