用单克隆抗体测定伪狂犬病病毒野毒株的抗原变异性
Determination of the antigenic variability of pseudorabies virus field isolates with monoclonal antibodies.
作者信息
Matisová J, Lesko J, Gallo J, Subr Z, Sabo A
机构信息
Institute of Virology, Slovak Academy of Sciences, Bratislava.
出版信息
Acta Vet Hung. 1994;42(2-3):343-6.
Five stable hybridoma cell lines secreting monoclonal antibodies (MAbs) were obtained by fusing spleen cells of pseudorabies virus (strain BUK) immunized BALB/c mice with mouse myeloma cell line SP2/0. Plate-trapped and antibody-trapped antigen ELISAs were done to compare the interaction of MAbs with TOP and "K" strains and 7 Slovak field isolates. Only 2 Slovak isolates reacted with all the MAbs, 3 MAbs reacted with TOP and "K" strains. One Slovak isolate gave no reaction with any MAbs.
通过将感染伪狂犬病病毒（BUK株）的BALB/c小鼠的脾细胞与小鼠骨髓瘤细胞系SP2/0融合，获得了5个分泌单克隆抗体（MAb）的稳定杂交瘤细胞系。进行了平板捕获和抗体捕获抗原ELISA，以比较单克隆抗体与TOP和“K”株以及7株斯洛伐克田间分离株的相互作用。只有2株斯洛伐克分离株与所有单克隆抗体发生反应，3种单克隆抗体与TOP和“K”株发生反应。1株斯洛伐克分离株与任何单克隆抗体均无反应。
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