Sharma R, Schumacher U, Adam E
Human Morphology, University of Southampton, Southampton, United Kingdom.
J Histochem Cytochem. 1998 Feb;46(2):143-8. doi: 10.1177/002215549804600202.
Peyer's patches in the intestinal mucosa are characterized by the presence of several lymphatic follicles and interfollicular T-cell regions. Luminal antigens are transported across the intestinal epithelium to stimulate the Peyer's patch pre-B-cells in the follicles that proliferate and migrate to distant sites. Evidence suggests that antigen priming of B-lymphocytes is responsible for the number and location of Peyer's patches during postnatal life, but little is known about the histogenesis of Peyer's patches and their overlying membranous (M) cells. To examine whether T- and B-lymphocytes in Peyer's patches have an influence on M-cell generation, we studied the development of Peyer's patches and M-cells in severe combined immunodeficient (SCID) mice reconstituted with bone marrow cells from normal syngeneic mice. Our experiments demonstrate that the donor bone marrow cells in the host scid mice repopulate to form single (primary) follicles and aggregates of lymphoid nodules, the Peyer's patches. Use of the lectins Anguilla anguilla (AAA) and Ulex europaeus I (UEA-I) as positive markers of mouse Peyer's patch M-cells revealed that M-cells develop in the dome epithelium overlying the single primary follicles and Peyer's patches of reconstituted scid mice. This experimental system therefore allows the study of the histogenesis of Peyer's patches and M-cells.
肠道黏膜中的派尔集合淋巴结的特征是存在多个淋巴滤泡和滤泡间T细胞区域。肠腔抗原穿过肠上皮细胞，刺激滤泡中的派尔集合淋巴结前B细胞，这些细胞增殖并迁移到远处。有证据表明，出生后B淋巴细胞的抗原启动决定了派尔集合淋巴结的数量和位置，但关于派尔集合淋巴结及其上方膜性（M）细胞的组织发生知之甚少。为了研究派尔集合淋巴结中的T淋巴细胞和B淋巴细胞是否对M细胞的生成有影响，我们研究了用同基因正常小鼠骨髓细胞重建的严重联合免疫缺陷（SCID）小鼠中派尔集合淋巴结和M细胞的发育情况。我们的实验表明，宿主scid小鼠中的供体骨髓细胞重新填充，形成单个（初级）滤泡和淋巴小结聚集体，即派尔集合淋巴结。使用鳗鲡凝集素（AAA）和荆豆凝集素I（UEA-I）作为小鼠派尔集合淋巴结M细胞的阳性标记物，发现M细胞在重建的scid小鼠单个初级滤泡和派尔集合淋巴结上方的圆顶上皮中发育。因此，这个实验系统可以用于研究派尔集合淋巴结和M细胞的组织发生。
