暴露于庆大霉素或妥布霉素期间猪肾（LLC-PK1）细胞膜的流动性
Pig kidney (LLC-PK1) cell membrane fluidity during exposure to gentamicin or tobramycin.
作者信息
Kohlhepp S J, Hou L, Gilbert D N
机构信息
Center for Fluorescence Research in Biomedical Sciences, Carnegie-Mellon University, Pittsburgh, Pennsylvania 15213.
出版信息
Antimicrob Agents Chemother. 1994 Sep;38(9):2169-71. doi: 10.1128/AAC.38.9.2169.
Abstract
The surface membrane properties of LLC-PK1 cells grown with and without various amounts of gentamicin or tobramycin for various lengths of time were determined by measuring the diffusion coefficient of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)dipalmitoyl-L- alpha-phosphatidylethanolamine (NBD-PE) and the percentage of NBD-PE free to diffuse after photobleaching. One hour of exposure to tobramycin decreased the percentage that was free to diffuse. After 1 day or longer of exposure to either aminoglycoside the percentage that was free to diffuse returned to preexposure levels and the diffusion coefficient decreased.
摘要
通过测量N-(7-硝基苯并-2-恶唑-1,3-二氮杂环丁烷-4-基)二棕榈酰-L-α-磷脂酰乙醇胺（NBD-PE）的扩散系数以及光漂白后可自由扩散的NBD-PE的百分比，来测定在添加或不添加不同量庆大霉素或妥布霉素的情况下培养不同时长的LLC-PK1细胞的表面膜特性。暴露于妥布霉素1小时会降低可自由扩散的百分比。在暴露于任何一种氨基糖苷类药物1天或更长时间后，可自由扩散的百分比恢复到暴露前水平，且扩散系数降低。
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本文引用的文献
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