对环磷酸腺苷受体蛋白结合位点与乳糖启动子之间间隔DNA的分析。
Analysis of the spacer DNA between the cyclic AMP receptor protein binding site and the lac promoter.
作者信息
Flatow U, Rajendrakumar G V, Garges S
机构信息
Developmental Genetics Section, Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892-4255, USA.
出版信息
J Bacteriol. 1996 Apr;178(8):2436-9. doi: 10.1128/jb.178.8.2436-2439.1996.
Abstract
The role of the spacer region DNA between the cyclic AMP receptor protein (CRP) site and the RNA polymerase in the lac promoter was examined. We wanted to determine whether the wild-type DNA sequence of this region was an absolute requirement for CRP activation of lac transcription. The sequence of a 9-bp stretch of the spacer, from -41 to -49 relative to the start of transcription, was randomized, and the effect of randomization on lac expression was investigated in vitro and in vivo. We found that the spacer contains no specific sequence determinants for CRP activation of lac transcription; fewer than 1% of the mutants displayed greater than a 50% decrease in CRP activation of lac transcription.
摘要
研究了环腺苷酸受体蛋白（CRP）位点与RNA聚合酶之间间隔区DNA在乳糖启动子中的作用。我们想确定该区域的野生型DNA序列是否是CRP激活乳糖转录的绝对必要条件。相对于转录起始点，间隔区一段9个碱基对的序列（从-41到-49）被随机化，并在体外和体内研究了随机化对乳糖表达的影响。我们发现，间隔区不包含CRP激活乳糖转录的特定序列决定因素；不到1%的突变体在CRP激活乳糖转录方面表现出超过50%的下降。
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