Kohno-Murase J, Murase M, Ichikawa H, Imamura J
Plantech Research Institute, Kanagawa, Japan.
Plant Mol Biol. 1994 Nov;26(4):1115-24. doi: 10.1007/BF00040693.
To manipulate the quantity and quality of storage components in Brassica napus seeds, we have constructed an antisense gene for the storage protein napin. The antisense gene was driven by the 5'-flanking region of the B. napus napin gene to express antisense RNA in a seed-specific manner. Seeds of transgenic plants with antisense genes often contained reduced amounts of napin. In some transgenic plants, no accumulation of napin was observed. However, the total protein content of transgenic and wild-type seeds did not differ significantly. Seeds lacking napin accumulated 1.4 to 1.5 times more cruciferin than untransformed seeds, although the oleosin content was not affected. Fatty acid content and composition in the seeds of transgenic plants were also analyzed by gas chromatography. Though the total fatty acid content of the transformants was the same as that of non-transformants, there was a reduction in 18:1 contents and a concomitant increase of 18:2 in seeds with reduced napin levels. This observed change in fatty acid composition was inherited in the next generation.
为了调控甘蓝型油菜种子中贮藏成分的数量和质量，我们构建了一个针对贮藏蛋白napin的反义基因。该反义基因由甘蓝型油菜napin基因的5'侧翼区驱动，以种子特异性方式表达反义RNA。携带反义基因的转基因植物种子中napin的含量通常会降低。在一些转基因植物中，未观察到napin的积累。然而，转基因种子和野生型种子的总蛋白含量没有显著差异。缺乏napin的种子中十字花科蛋白的积累量比未转化种子多1.4至1.5倍，不过油质蛋白的含量不受影响。还通过气相色谱法分析了转基因植物种子中的脂肪酸含量和组成。尽管转化体的总脂肪酸含量与非转化体相同，但在napin水平降低的种子中，18:1含量减少，同时18:2含量增加。这种观察到的脂肪酸组成变化在下一代中得以遗传。
