Indian J Microbiol. 2011 Jun;51(2):176-81. doi: 10.1007/s12088-011-0129-y. Epub 2011 Jan 25.
We report the production of two types of siderophores namely catecholate and hydroxamate in modified succinic acid medium (SM) from Alcaligenes faecalis. Two fractions of siderophores were purified on amberlite XAD, major fraction was hydroxamate type having a λ(max) at 224 nm and minor fraction appeared as catecholate with a λ(max) of 264 nm. The recovery yield obtained from major and minor fractions was 297 and 50 mg ml(-1) respectively. The IEF pattern of XAD-4 purified siderophore suggested the pI value of 6.5. Cross feeding studies revealed that A. faecalis accepts heterologous as well as self (hydroxamate) siderophore in both free and iron complexed forms however; the rate of siderophore uptake was more in case of siderophores complexed to iron. Siderophore iron uptake studies indicated the differences between hydroxamate siderophore of A. faecalis and Alc E, a siderophore of Alcaligenes eutrophus.
我们报告了两种类型的铁载体即儿茶酚和羟肟酸在经过改良的琥珀酸培养基 (SM) 中由粪产碱杆菌产生。两种铁载体的级分在 Amberlite XAD 上进行了纯化，主要级分是羟肟酸盐型，其 λ(max) 在 224nm 处，次要级分呈现出儿茶酚盐型，λ(max)为 264nm。主要级分和次要级分的回收产率分别为 297 和 50mg/ml。XAD-4 纯化的铁载体的等电聚焦图谱表明其等电点为 6.5。交叉喂养研究表明，粪产碱杆菌既接受同源的（羟肟酸盐）铁载体，也接受异源（包括游离形式和铁络合形式）的铁载体；然而，在铁载体与铁络合的情况下，铁载体的摄取速率更高。铁载体铁摄取研究表明了粪产碱杆菌的羟肟酸盐铁载体与 Alcaligenes eutrophus 的 Alc E 铁载体之间的差异。
