Vendeland S C, Deagen J T, Butler J A, Whanger P D
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
Biometals. 1994 Oct;7(4):305-12. doi: 10.1007/BF00144126.
The uptake of selenite, selenate and selenomethionine (SeMet) was performed with brush border membrane vesicles (BBMV) prepared from rats fed selenium-deficient and supplemented diets. At equilibrium (60 min), the uptake of 75Se from [75Se]selenite ranged from 16.5 to 18.9 nmol mg-1 protein. There was a curvilinear relationship in the uptake of selenite over a concentration range of 10-1000 microM. About 2 nmol mg-1 protein was obtained with selenomethionine (SeMet) which occurred between 90 and 180 s. In contrast to selenite, there was a linear relationship in the initial uptake of SeMet over a concentration range of 10-1000 microM. The uptake of selenate was approximately 50-fold lower than selenite, reaching 350 pmol mg-1 protein. Dietary selenium level had no effect on the rate of 75Se accumulation by BBMV. Dramatic differences are found in the uptake and binding of selenium by BBMV incubated with different selenocompounds.
用从喂食缺硒和补硒日粮的大鼠制备的刷状缘膜囊泡（BBMV）进行亚硒酸盐、硒酸盐和硒代蛋氨酸（SeMet）的摄取实验。在平衡状态（60分钟）时，[75Se]亚硒酸盐中75Se的摄取量为16.5至18.9 nmol mg-1蛋白质。在10 - 1000 microM的浓度范围内，亚硒酸盐的摄取呈曲线关系。硒代蛋氨酸（SeMet）在90至180秒之间摄取量约为2 nmol mg-1蛋白质。与亚硒酸盐不同，在10 - 1000 microM的浓度范围内，SeMet的初始摄取呈线性关系。硒酸盐的摄取量比亚硒酸盐低约50倍，达到350 pmol mg-1蛋白质。日粮硒水平对BBMV积累75Se的速率没有影响。在用不同硒化合物孵育的BBMV中，发现硒的摄取和结合存在显著差异。
