Sourek J, Oravec C
National Institute of Public Health, Prague, Czech Republic.
Cent Eur J Public Health. 1994 Jun;2(1):62-5.
The tumoricidal effect of endotoxins and their subunits of Shigella dysenteriae serovar 1 of both growth forms and certain other representatives of the Enterobacteriaceae family was tested against Németh-Kellner mouse lymphoma cells using an in vitro assay based on the use of sodium chromate solution yielding labelled hexavalent 51Cr ions. The most effective in vitro activity was evidenced in both growth forms by S. dysenteriae 1 lipopolysaccharide-protein complex (LPSP) (76-92%), lipid A and lipoid B isolated from LPS (77-82%) and lipid A and lipoid B from LPSP (53-70%). A direct dependence of the level of the Limulus test and pyrogenicity on the tumoricidal activity of a preparation was not demonstrated. The influence of selected cations (Cu, Fe, Ca, Mg, Zn) bound to selected substances on antitumor activity was monitored. The method of probit analysis is recommended as it enables estimation, based on a number of concentrations, of the regression line of probable effectiveness of a given preparation.
使用基于铬酸钠溶液产生标记的六价51Cr离子的体外试验，测试了痢疾志贺氏菌血清型1的内毒素及其两种生长形式的亚基以及肠杆菌科的某些其他代表对Németh-Kellner小鼠淋巴瘤细胞的杀肿瘤作用。痢疾志贺氏菌1脂多糖-蛋白质复合物（LPSP）（76-92%）、从LPS分离的脂质A和类脂B（77-82%）以及LPSP中的脂质A和类脂B（53-70%）在两种生长形式中均表现出最有效的体外活性。未证明鲎试剂水平和致热原性与制剂杀肿瘤活性之间存在直接相关性。监测了与选定物质结合的选定阳离子（铜、铁、钙、镁、锌）对抗肿瘤活性的影响。推荐使用概率分析方法，因为它能够基于多个浓度估计给定制剂可能有效性的回归线。
