Francés V, Pandrau-Garcia D, Guret C, Ho S, Wang Z, Duvert V, Saeland S, Martinez-Valdez H
Schering-Plough, Laboratory for Immunology Research, Dardilly, France.
EMBO J. 1994 Dec 15;13(24):5937-43. doi: 10.1002/j.1460-2075.1994.tb06939.x.
A novel kappa protein, encoded by a germline JC kappa transcript, is expressed by normal and leukemic human B cell precursors. The transcript displays an open reading frame initiated by a non-AUG codon, and predicts a 15 kDa molecule which could be readily confirmed by in vitro translation. Cellular expression was demonstrated by immunofluorescence, precipitation and Western blotting. Furthermore, 2-D gel electrophoresis revealed that germline JC kappa can covalently associate with mu heavy chain at the surface of pre-B cells. We therefore propose that during B cell lymphopoiesis, two alternative pathways could be operative in which mu heavy chain can either associate with lambda 5 or germ-line JC kappa.
一种由种系JC κ转录本编码的新型κ蛋白，由正常和白血病人类B细胞前体表达。该转录本显示出由非AUG密码子起始的开放阅读框，并预测出一个15 kDa的分子，这可以通过体外翻译很容易地得到证实。通过免疫荧光、沉淀和蛋白质印迹法证实了细胞表达。此外，二维凝胶电泳显示种系JC κ可以在pre-B细胞表面与μ重链共价结合。因此，我们提出在B细胞淋巴细胞生成过程中，可能存在两种替代途径，其中μ重链可以与λ5或种系JC κ结合。
