Carter D C, Chang B, Ho J X, Keeling K, Krishnasami Z
Biophysics Branch, Marshall Space Flight Center, Alabama 35812.
Eur J Biochem. 1994 Dec 15;226(3):1049-52. doi: 10.1111/j.1432-1033.1994.01049.x.
Several crystal forms of serum albumin suitable for three-dimensional structure determination have been grown. These forms include crystals of recombinant and wild-type human serum albumin, baboon serum albumin, and canine serum albumin. The intrinsic limits of X-ray diffraction for these crystals are in the range 0.28-0.22 nm. Two of the crystal forms produced from human and canine albumin include incorporated long-chain fatty acids. Molecular replacement experiments have been successfully conducted on each crystal form using the previously determined atomic coordinates of human serum albumin illustrating the conserved tertiary structure.
已经培养出了几种适合用于三维结构测定的血清白蛋白晶体形式。这些形式包括重组型和野生型人血清白蛋白、狒狒血清白蛋白以及犬血清白蛋白的晶体。这些晶体的X射线衍射内在极限在0.28 - 0.22纳米范围内。由人和犬白蛋白产生的两种晶体形式包含掺入的长链脂肪酸。利用先前确定的人血清白蛋白原子坐标，已在每种晶体形式上成功进行了分子置换实验，这说明了三级结构的保守性。
