Schweisguth F, Nero P, Posakony J W
Institut Jacques Monod, CNRS-Université Paris VII, France.
Dev Biol. 1994 Dec;166(2):812-4. doi: 10.1006/dbio.1994.1359.
Suppressor of Hairless [Su(H)] plays an essential role in neurogenesis in Drosophila by controlling successive alternative cell fate decisions in the developing adult epidermis. Analysis of the predicted amino acid sequence of the Su(H) protein revealed a weak similarity to the catalytic domain of a family of phage integrases and yeast recombinases. We present here the results of a site-directed mutagenesis of the integrase-related region of Su(H), which indicate that this sequence similarity has no functional significance in vivo. We suggest that the JK-RBP protein, encoded by the mouse homologue of Su(H), does not act as a recombinase, as originally proposed.
无毛抑制因子[Su(H)]通过控制发育中的成年表皮中连续的替代细胞命运决定，在果蝇的神经发生中发挥着至关重要的作用。对Su(H)蛋白预测的氨基酸序列分析显示，它与噬菌体整合酶和酵母重组酶家族的催化结构域有微弱的相似性。我们在此展示了对Su(H)整合酶相关区域进行定点诱变的结果，这些结果表明这种序列相似性在体内没有功能意义。我们认为，由Su(H)的小鼠同源物编码的JK-RBP蛋白并不像最初所提出的那样作为一种重组酶发挥作用。
