Prashar R, Kumar A
Department of Zoology, University of Rajasthan, Jaipur, India.
Indian J Exp Biol. 1994 Sep;32(9):643-6.
DMBA (195 nmol/50 microliters of acetone/animal) was applied topically over the dorsal skin of the mice and tumors were promoted by repeated applications of croton oil (1% in acetone, three times per week) after two weeks of DMBA application. Skin papillomas appeared in 100% animals in control as well as in groups treated orally with Liv. 52 at post-initiational stages and continuously at peri-initiational and post-initiational stages of papillomagenesis. When Liv. 52 was given orally at the peri-initiational stage of papillomagenesis, the percentage of mice bearing tumors was 75% and the tumor mean per mouse was reduced to 4.0 +/- 1.63 as compared to 7.5 +/- 3.54 in the control group after 15 weeks of observation. The tumor mean per mouse was observed to be 4.75 +/- 0.55 and 2.5 +/- 0.57 in the groups treated orally with Liv. 52 at the post-initiational stages and continuously at peri-initiational and post-initiational stages of papillomagenesis respectively. Similarly, the cumulative number of papillomas after 15 weeks was 30 in the control group, which was reduced to 10 in the animals treated with Liv. 52 continuously at peri-initiational and post-initiational stages. The cumulative number of papillomas was also reduced to 16 and 19 in animals treated with Liv. 52 at peri-initiational and post-initiational stages, respectively.
将二甲基苯蒽（195纳摩尔/50微升丙酮/只动物）局部涂抹于小鼠背部皮肤，在涂抹二甲基苯蒽两周后，通过反复涂抹巴豆油（丙酮中1%，每周三次）来促进肿瘤形成。在启动后阶段以及在乳头瘤发生的启动期和启动后阶段持续口服利肝52的各组动物中，100%出现了皮肤乳头瘤。在乳头瘤发生的启动期口服利肝52时，观察15周后，患肿瘤小鼠的百分比为75%，每只小鼠的肿瘤平均数降至4.0±1.63，而对照组为7.5±3.54。在启动后阶段口服利肝52以及在乳头瘤发生的启动期和启动后阶段持续口服利肝52的组中，每只小鼠的肿瘤平均数分别观察为4.75±0.55和2.5±0.57。同样，15周后对照组的乳头瘤累积数为30个，在启动期和启动后阶段持续用利肝52治疗的动物中减少至10个。在启动期和启动后阶段用利肝52治疗的动物中，乳头瘤累积数也分别减少至16个和19个。
