van Etten L, Folman C C, Eggelte T A, Kremsner P G, Deelder A M
Laboratory of Parasitology, Medical Faculty, University of Leiden, Germany.
J Clin Microbiol. 1994 Oct;32(10):2404-6. doi: 10.1128/jcm.32.10.2404-2406.1994.
For the rapid and simple diagnosis of schistosomiasis, a reagent strip assay for detection of circulating cathodic antigen (CCA) in urine was developed. The test was based on a previously developed sandwich enzyme-linked immunosorbent assay (ELISA) using a combination of two anti-CCA monoclonal antibodies. For the reagent strip assay, as the capture matrix, monoclonal antibody was coated onto a nitrocellulose membrane and mounted on polyvinyl chloride strips. Urine samples were then tested in an assay consisting of a combined incubation step of the urine sample and biotinylated detecting antibody followed by incubation in streptavidin peroxidase and a subsequent staining. The specificity and the sensitivity of the assay, as determined with urine samples of 61 uninfected controls and 67 Schistosoma mansoni-infected individuals, were 96.7 and 95.5%, respectively. The results of the reagent strip assay compared very well with microscopical parasitological diagnosis by the standard Kato-Katz method for the same individuals. The reagent strip test has a lower detection limit of 1 ng of CCA per ml and can be completed in 75 min. By the inclusion of two reference bands on the strips, standardized reading could be achieved. This reagent strip assay is a promising tool for qualitative diagnosis of S. mansoni infections in control programs.
为实现血吸虫病的快速简易诊断，研发了一种用于检测尿液中循环阴极抗原（CCA）的试剂条检测法。该检测基于先前开发的夹心酶联免疫吸附测定法（ELISA），使用了两种抗CCA单克隆抗体的组合。对于试剂条检测，将单克隆抗体作为捕获基质包被在硝酸纤维素膜上，并安装在聚氯乙烯条上。然后，在一个检测中对尿液样本进行检测，该检测包括尿液样本与生物素化检测抗体的联合孵育步骤，随后在链霉亲和素过氧化物酶中孵育并进行后续染色。用61名未感染对照和67名曼氏血吸虫感染个体的尿液样本测定，该检测的特异性和敏感性分别为96.7%和95.5%。对于相同个体，试剂条检测结果与标准加藤厚涂片法的显微镜寄生虫学诊断结果非常吻合。试剂条检测的检测下限为每毫升1纳克CCA，可在75分钟内完成。通过在试纸上设置两条参考带，可实现标准化读数。这种试剂条检测法是控制项目中曼氏血吸虫感染定性诊断的一种有前景的工具。
