Krijger F W, van Lieshout L, Deelder A M
Laboratory of Parasitology, Medical Faculty, University of Leiden, The Netherlands.
Acta Trop. 1994 Feb;56(1):55-63. doi: 10.1016/0001-706x(94)90040-x.
For the detection of the circulating schistosome antigens CAA (circulating anodic antigen) and CCA (circulating cathodic antigen) in serum and urine samples of Schistosoma infected individuals, pretreatment of samples with trichloroacetic acid (TCA) is a standard procedure. In the present study several methods were evaluated in order to develop a more simple and rapid technique than the--especially for pretreatment of urine samples--laborious TCA technique. Optimal results were obtained with a method in which serum or urine samples were pretreated by a heat-incubation step (70 degrees C, 30 min) in an alkaline buffer (pH 9.6). In a comparison of the new technique with the TCA pretreatment, serum and urine samples of S. mansoni infected individuals from Zaire (n = 80) and of uninfected controls from The Netherlands (n = 208) were pretreated and assayed for CAA and CCA. Both pretreatment techniques showed similar sensitivities and specificities for CAA and CCA in serum, and CCA in urine. However, for the determination of CAA in urine the new technique performed significantly better, resulting in an increase of the sensitivity from 32 to 70% (titre determination).
为检测血吸虫感染个体血清和尿液样本中的循环血吸虫抗原CAA（循环阳极抗原）和CCA（循环阴极抗原），用三氯乙酸（TCA）对样本进行预处理是一种标准程序。在本研究中，评估了几种方法，以开发一种比——尤其是对于尿液样本预处理——繁琐的TCA技术更简单快速的技术。通过一种方法获得了最佳结果，即血清或尿液样本在碱性缓冲液（pH 9.6）中经热孵育步骤（70℃，30分钟）进行预处理。在将新技术与TCA预处理进行比较时，对来自扎伊尔的曼氏血吸虫感染个体的血清和尿液样本（n = 80）以及来自荷兰的未感染对照的样本（n = 208）进行预处理，并检测CAA和CCA。两种预处理技术对血清中的CAA和CCA以及尿液中的CCA均显示出相似的敏感性和特异性。然而，对于尿液中CAA的测定，新技术表现明显更好，导致敏感性从32%提高到70%（滴度测定）。
