Ross H J, Antoniono R J, Buckmeier J A, Redpath J L
Department of Medicine, College of Medicine, University of California at Irvine 92717.
Int J Radiat Biol. 1994 Dec;66(6):785-91.
Interleukin 1 (IL-1) has been reported to act as a radioprotector in vivo. Data from our laboratory and from other investigators suggest that glioma cell lines can produce bioactive cytokines including IL-1 and also express IL-1 receptors. In view of the putative radioprotective effect of this cytokine, we have examined the in vitro radiosensitivity of three human glioma cell lines with widely varying levels of endogenous IL-1 beta. The data reveal that when irradiated (2 Gy/min) as confluent cultures (conditions optimal for differential IL-1 beta expression), and plated for colony formation after postirradiation holding, cell survival was not correlated with level of IL-1 beta mRNA expression in the two IL-1-expressing cell lines. However, this was not correlated with a further reduction in radiosensitivity. These data indicate that IL-1 beta does not act as an endogenous radioprotector in these cells under these experimental conditions.
白细胞介素1（IL-1）据报道在体内可作为一种辐射防护剂。我们实验室以及其他研究者的数据表明，胶质瘤细胞系能够产生包括IL-1在内的生物活性细胞因子，并且也表达IL-1受体。鉴于这种细胞因子假定的辐射防护作用，我们检测了三种内源性IL-1β水平差异很大的人胶质瘤细胞系的体外放射敏感性。数据显示，当作为汇合培养物（这是差异表达IL-1β的最佳条件）以2 Gy/分钟的剂量进行照射，并在照射后孵育后接种以形成集落时，在两个表达IL-1的细胞系中，细胞存活与IL-1β mRNA表达水平无关。然而，这与放射敏感性的进一步降低并无关联。这些数据表明，在这些实验条件下，IL-1β在这些细胞中并不作为内源性辐射防护剂发挥作用。
