Strätling W H
Nucleic Acids Res. 1976 May;3(5):1203-13. doi: 10.1093/nar/3.5.1203.
Polar organic compounds, including DMSO, increase RNA synthesis on isolated chromatin by E. coli RNA polymerase and RNA polymerase II from calf thymus. Transcription is stimulated on chromatin from Friend-virus-infected erythroleukemia cells and from various other sources. Using procedures which inhibit specifically the formation of a stable initiation complex, it is shown that the stimulation does not result from an increase in initiation of both E. coli and the eukaryotic RNA polymerase. After separation of chromatin into template active and inactive fractions, DMSO increases RNA synthesis by a factor of about 1.5 using the template inactive fraction, while stimulation of transcription on the template active portion is lower (factor of 1.2). It is suggested that the effect on RNA synthesis is mediated by a weakening of the apolar interactions between histones in chromatin subunits, releasing transcription partially from the constraints imposed by histones.
包括二甲基亚砜（DMSO）在内的极性有机化合物，可增强大肠杆菌RNA聚合酶和小牛胸腺RNA聚合酶II对分离染色质的RNA合成作用。在来自Friend病毒感染的红白血病细胞及其他各种来源的染色质上，转录均受到刺激。采用特异性抑制稳定起始复合物形成的方法表明，这种刺激并非源于大肠杆菌和真核RNA聚合酶起始作用的增加。将染色质分离为模板活性和非活性部分后，DMSO使用模板非活性部分可使RNA合成增加约1.5倍，而对模板活性部分转录的刺激较低（1.2倍）。有人提出，对RNA合成的影响是通过减弱染色质亚基中组蛋白之间的非极性相互作用来介导的，从而使转录部分地从组蛋白施加的限制中释放出来。
