Gutiérrez L M, Viniegra S, Quintanar J L, Reig J A, Sala F
Departamento de Neuroquímica, Universidad de Alicante, Spain.
Neurosci Lett. 1994 Aug 29;178(1):55-8. doi: 10.1016/0304-3940(94)90288-7.
The specific phosphatase inhibitor, Calyculin-A (CL-A), decreases high-K stimulated catecholamine secretion in bovine chromaffin cells. This effect can be split into two components: one needs long exposures to the drug to be elicited, and is sensitive to the protein kinase-inhibitor K252a; the other is observed after short incubations of CL-A, and is insensitive to K252a. Here we report that the latter component is due to an external block, by CL-A, of chromaffin cell calcium channels in a voltage-dependent, reversible and phosphorylation-independent manner.
特异性磷酸酶抑制剂，花萼海绵诱癌素-A（CL-A），可减少高钾刺激的牛嗜铬细胞中儿茶酚胺的分泌。这种效应可分为两个部分：一部分需要长时间暴露于该药物才能引发，并且对蛋白激酶抑制剂K252a敏感；另一部分在CL-A短期孵育后即可观察到，并且对K252a不敏感。在此我们报告，后一部分是由于CL-A以电压依赖性、可逆且不依赖磷酸化的方式对外周嗜铬细胞钙通道进行阻断所致。
