Lygerou Z, Conesa C, Lesage P, Swanson R N, Ruet A, Carlson M, Sentenac A, Séraphin B
EMBL, Heidelberg, Germany.
Nucleic Acids Res. 1994 Dec 11;22(24):5332-40. doi: 10.1093/nar/22.24.5332.
While screening for genes that affect the synthesis of yeast snRNPs, we identified a thermosensitive mutant that abolishes the production of a reporter snRNA at the non-permissive temperature. This mutant defines a new gene, named BDF1. In a bdf1-1 strain, the reporter snRNA synthesized before the temperature shift remains stable at the non-permissive temperature. This demonstrates that the BDF1 gene affects the synthesis rather than the stability of the reporter snRNA and suggests that the BDF1 gene encodes a transcription factor. BDF1 is present in single copy on yeast chromosome XII, and is important for normal vegetative growth but not essential for cell viability. bdf1 null mutants share common phenotypes with several mutants affecting general transcription and are defective in snRNA production. BDF1 encodes a protein of 687 amino-acids containing two copies of the bromodomain, a motif also present in other transcription factors as well as a new conserved domain, the ET domain, also present in Drosophila and human proteins.
在筛选影响酵母小核核糖核蛋白（snRNP）合成的基因时，我们鉴定出一个温度敏感型突变体，该突变体在非允许温度下会消除报告小核RNA（snRNA）的产生。这个突变体定义了一个新基因，命名为BDF1。在bdf1-1菌株中，温度转换前合成的报告snRNA在非允许温度下保持稳定。这表明BDF1基因影响报告snRNA的合成而非稳定性，并提示BDF1基因编码一种转录因子。BDF1以单拷贝形式存在于酵母的第十二号染色体上，对正常的营养生长很重要，但对细胞活力并非必不可少。bdf1缺失突变体与几个影响一般转录的突变体具有共同的表型，并且在snRNA产生方面存在缺陷。BDF1编码一个含687个氨基酸的蛋白质，该蛋白质包含两个溴结构域拷贝，这是一种也存在于其他转录因子中的基序，以及一个新的保守结构域，即ET结构域，该结构域也存在于果蝇和人类蛋白质中。
