一种用于鉴定来自牛乳腺炎的凝固酶阴性葡萄球菌的简易方法。
A simple identification scheme for coagulase negative staphylococci from bovine mastitis.
作者信息
Devriese L A, Laevens H, Haesebrouck F, Hommez J
机构信息
Faculty of Veterinary Medicine, University of Ghent, Belgium.
出版信息
Res Vet Sci. 1994 Sep;57(2):240-4. doi: 10.1016/0034-5288(94)90064-7.
Coagulase-negative staphylococci from cases of bovine mastitis were identified to species level by using an identification scheme based on a three-plate test system which tested for DNase on DNA agar, for protease on calcium caseinate agar, and for the organism's sensitivity to novobiocin, desferrioxaminine (deferoxamine) and fosfomycin by agar diffusion tests. Testing for the inhibition of Staphylococcus delta haemolysin (Skalka 1991) can replace the protease tests.
通过使用基于三板测试系统的鉴定方案，将来自牛乳腺炎病例的凝固酶阴性葡萄球菌鉴定到种水平。该测试系统包括在DNA琼脂上检测DNase、在酪蛋白钙琼脂上检测蛋白酶，以及通过琼脂扩散试验检测该微生物对新生霉素、去铁胺和磷霉素的敏感性。检测δ-溶血葡萄球菌抑制作用（Skalka，1991）可替代蛋白酶检测。
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