凝固酶阳性葡萄球菌鉴别方法的评估
Evaluation of methods for differentiation of coagulase-positive staphylococci.
作者信息
Roberson J R, Fox L K, Hancock D D, Besser T E
机构信息
Department of Veterinary Clinical Medicine and Surgery, Washington State University, Pullman 99164-6610.
出版信息
J Clin Microbiol. 1992 Dec;30(12):3217-9. doi: 10.1128/jcm.30.12.3217-3219.1992.
The purpose of this study was to determine the minimum number of tests that could be used to differentiate between the coagulase-positive strains of staphylococcus: Staphylococcus aureus, Staphylococcus hyicus, and Staphylococcus intermedius. Eighty coagulase-positive strains of each of the three species were examined. The five tests conducted were growth on modified Baird-Parker agar, growth on P agar supplemented with acriflavin, production of acetoin, anaerobic fermentation of mannitol, and presence of beta-galactosidase. Positive test percentages for S. aureus were 100% for growth on modified Baird-Parker agar, 100% for growth on P agar supplemented with acriflavin, 94% for production of acetoin, 99% for anaerobic fermentation of mannitol, and 0% for presence of beta-galactosidase. Positive test percentages for S. intermedius were 0% for growth on modified Baird-Parker agar, 0% for growth on P agar supplemented with acriflavin, 1% for production of acetoin, 0% for anaerobic fermentation of mannitol, and 100% for presence of beta-galactosidase. S. hyicus isolates were negative in all five tests. Results from the 240 coagulase-positive staphylococcus strains tested would suggest correct identification of coagulase-positive staphylococci with P agar supplemented with acriflavin and the beta-galactosidase test. These two tests are simple to conduct and result in quick and easy differentiation of the three coagulase-positive staphylococcal species.
本研究的目的是确定可用于区分凝固酶阳性葡萄球菌菌株的最少测试数量
金黄色葡萄球菌、猪葡萄球菌和中间葡萄球菌。对这三个菌种的80株凝固酶阳性菌株进行了检测。进行的五项测试分别是在改良的贝尔德-帕克琼脂上生长、在添加吖啶黄素的P琼脂上生长、产乙酰甲基甲醇、甘露醇厌氧发酵以及β-半乳糖苷酶的存在情况。金黄色葡萄球菌在改良的贝尔德-帕克琼脂上生长的阳性测试百分比为100%，在添加吖啶黄素的P琼脂上生长的阳性测试百分比为100%，产乙酰甲基甲醇的阳性测试百分比为94%，甘露醇厌氧发酵的阳性测试百分比为99%，β-半乳糖苷酶存在情况的阳性测试百分比为0%。中间葡萄球菌在改良的贝尔德-帕克琼脂上生长的阳性测试百分比为0%，在添加吖啶黄素的P琼脂上生长的阳性测试百分比为0%，产乙酰甲基甲醇的阳性测试百分比为1%，甘露醇厌氧发酵的阳性测试百分比为0%，β-半乳糖苷酶存在情况的阳性测试百分比为100%。猪葡萄球菌分离株在所有五项测试中均为阴性。对240株凝固酶阳性葡萄球菌菌株的检测结果表明，使用添加吖啶黄素的P琼脂和β-半乳糖苷酶测试可正确鉴定凝固酶阳性葡萄球菌。这两项测试操作简单，能快速轻松地区分三种凝固酶阳性葡萄球菌菌种。
Zotero 插件