Nolte I, Heyde P, Mischke R, Müller-Berghaus G
Clinic of Small Animals, Hanover, School of Veterinary Medicine, Germany.
Zentralbl Veterinarmed A. 1994 Jun;41(5):385-95. doi: 10.1111/j.1439-0442.1994.tb00105.x.
The aim of the present study was to test the haemostyptic properties of a phospholipid complex with platelet factor-3 (PF-3) activity in platelet-dependent haemostatic disorders. The substance was investigated in an animal model comprising six investigational groups (five dogs each). A thrombocytopathy was created in the dogs belonging to groups 1-5 by intravenous administration of 20 mg/kg body weight (BW) acetylsalicylic acid (ASA). Two hours later a human albumin solution (5%) (control group) or a phospholipid complex with PF-3 activity was injected or infused intravenously in different dosages. In dogs pre-treated with ASA, injection of 2 ml/kg BW of the phospholipid complex shortened capillary bleeding time, which was prolonged as a consequence of ASA-treatment. This effect lasted for 4 h at least. The capability of the platelets to aggregate increased 5 min after intravenous injection of the phospholipid without differences in the respective groups. At the same time platelet counts dropped to approximately 50%, but increased again distinctly after 30 min. When the phospholipid complex was administered to clinically healthy dogs who had not been treated with ASA, this resulted in prolongation of the capillary bleeding time as well as a significant platelet drop in the peripheral blood. Although these were only short-term effects after administration of the phospholipid complex, a disadvantageous effect cannot be excluded in patients suffering from haemorrhagic diathesis. For this reason, a phospholipid complex with PF-3 activity cannot be recommended as a therapeutic agent for platelet-dependent coagulation disorders in the dog.
本研究的目的是在血小板依赖性止血障碍中测试具有血小板因子3（PF - 3）活性的磷脂复合物的止血特性。该物质在一个包含六个研究组（每组五只狗）的动物模型中进行了研究。通过静脉注射20mg/kg体重的乙酰水杨酸（ASA），在第1 - 5组的狗中造成血小板病。两小时后，静脉注射或输注不同剂量的人白蛋白溶液（5%）（对照组）或具有PF - 3活性的磷脂复合物。在预先用ASA处理的狗中，注射2ml/kg体重的磷脂复合物缩短了因ASA处理而延长的毛细血管出血时间。这种效果至少持续4小时。静脉注射磷脂后5分钟，血小板聚集能力增强，各相应组之间无差异。与此同时，血小板计数下降至约50%，但30分钟后又明显回升。当将磷脂复合物给予未用ASA处理的临床健康狗时，这导致毛细血管出血时间延长以及外周血中血小板显著减少。尽管这些只是给予磷脂复合物后的短期效应，但不能排除对患有出血素质的患者产生不利影响。因此，不推荐将具有PF - 3活性的磷脂复合物作为狗血小板依赖性凝血障碍的治疗药物。
