Abdel-Naser M B, Blume-Peytavi U, Abdel-Fatah A, Orfanos C E
Department of Dermatology, University Medical Center Steglitz, Free University of Berlin, Germany.
Acta Derm Venereol. 1994 Sep;74(5):351-4. doi: 10.2340/0001555574351354.
Immunological mechanisms have long been suggested to mediate hair loss in alopecia areata. In this process hair bulb melanocytes and dermal papilla fibroblasts are believed to be primarily involved. In the present study we further investigated the role of humoral factors in alopecia areata. Three different experiments were performed on normal human epidermal melanocytes as well as normal human dermal fibroblasts: (i) incubation with medium containing 2, 10, or 20% alopecia areata serum (n = 12 patients) for 16 h, (ii) incubation with medium supplemented with preheated alopecia areata serum (1 h at 56 degrees C) and healthy human fresh serum as a complement source (1:1) and (iii) incubation with 2, 10 or 20% alopecia areata serum but, in addition, containing peripheral blood mononuclear cells from healthy subjects (effector/target ratio, 50:1). As controls, normal human fibroblasts and normal human epidermal melanocyte cultures were also incubated with serum from healthy individuals (n = 5) under the same culture conditions. The results showed that alopecia areata serum exerted a significant stimulation of proliferation of both normal human fibroblasts (p > 0.05 at 2%, p > 0.05 at 10%, p < 0.05 at 20%), and normal human epidermal melanocytes (p > 0.05 at 2%, p < 0.05 at 10%, p > 0.05 at 20%).(ABSTRACT TRUNCATED AT 250 WORDS)
长期以来，免疫机制一直被认为在斑秃脱发过程中起介导作用。在此过程中，毛囊黑素细胞和真皮乳头成纤维细胞被认为是主要参与细胞。在本研究中，我们进一步研究了体液因子在斑秃中的作用。我们对正常人表皮黑素细胞和正常人真皮成纤维细胞进行了三项不同的实验：（i）用含有2%、10%或20%斑秃血清（n = 12例患者）的培养基孵育16小时；（ii）用补充有预热斑秃血清（56℃孵育1小时）和健康人新鲜血清作为补体来源（1:1）的培养基孵育；（iii）用2%、10%或20%斑秃血清孵育，但此外还含有健康受试者的外周血单个核细胞（效应细胞/靶细胞比例为50:1）。作为对照，正常人成纤维细胞和正常人表皮黑素细胞培养物也在相同培养条件下用健康个体的血清（n = 5）孵育。结果表明，斑秃血清对正常人成纤维细胞（2%时p > 0.05，10%时p > 0.05，20%时p < 0.05）和正常人表皮黑素细胞（2%时p > 0.05，10%时p < 0.05，20%时p > 0.05）的增殖均有显著刺激作用。（摘要截短至250字）
