Säily M, Koistinen P, Laine S, Soppi E, Sovolainen E R
Department of Internal Medicine, University of Oulu, Finland.
Acta Haematol. 1994;92(2):71-8. doi: 10.1159/000204178.
The immunoglobulin (Ig) isotype (IgG, IgA, IgM) production of peripheral blood mononuclear cells from 28 patients having multiple myeloma (MM) was analyzed. The total Ig secreting capacity of the cells, as measured by ELISA from the cell culture medium, was not found to be significantly reduced in MM (1,118 +/- 1,394 micrograms/l) as compared to the values of 9 controls (898 +/- 520 micrograms/l), but a significant isotype switching towards the tumor paraprotein type was observed in the patients with active MM (p < 0.001). The percentage of IgG in the active IgG-MM was 88 +/- 11% and that of IgA in the active IgA-MM 83 +/- 13%, the control values being 44 +/- 11% for IgG and 44 +/- 13% for IgA. The proportions of isotypes resembled those of the controls in the inactive phase of the disease. Despite this dominating paraprotein class isotype production, no evidence of Ig gene clonal rearrangements was found in cells studied by either Southern blotting or the more sensitive polymerase chain reaction method, which suggests that polyclonal rather than monoclonal PB B cells are responsible for the Ig production observed.
分析了28例多发性骨髓瘤（MM）患者外周血单个核细胞的免疫球蛋白（Ig）同种型（IgG、IgA、IgM）产生情况。通过ELISA检测细胞培养基中细胞的总Ig分泌能力，发现MM患者（1,118±1,394微克/升）与9名对照者（898±520微克/升）相比，并未显著降低，但在活动性MM患者中观察到明显向肿瘤副蛋白类型的同种型转换（p<0.001）。活动性IgG-MM中IgG的百分比为88±11%，活动性IgA-MM中IgA的百分比为83±13%，对照者IgG的百分比为44±11%，IgA为44±13%。在疾病的非活动期，同种型比例与对照者相似。尽管存在这种占主导地位的副蛋白类别同种型产生，但通过Southern印迹法或更敏感的聚合酶链反应方法研究的细胞中未发现Ig基因克隆重排的证据，这表明多克隆而非单克隆外周血B细胞是所观察到的Ig产生的原因。
