Säily M, Koistinen P, Laine S, Soppi E, Savolainen E R
Department of Clinical Chemistry, University of Oulu, Finland.
Eur J Haematol. 1996 Aug;57(2):171-7. doi: 10.1111/j.1600-0609.1996.tb01356.x.
To test a hypothesis that interferon-alpha (IFN) treatment might restore normal immunoglobulin (Ig) production in multiple myeloma (MM), the effect of IFN on Ig isotype (IgG and IgA) production by peripheral blood (PB) and bone marrow (BM) mononuclear cells (MNCs) in MM patients was analyzed by ELISA. IFN at a concentration of 1000 U/ml was found to enhance IgA production by PB MNCs in IgA-MM and had a trend to stimulate IgG production in IgG-MM. The effect of IFN on nonparaprotein Ig isotype production was more variable, with mostly neutral or inhibitory effects being seen in both the MM subtypes. In contrast to the influences observed in MM patients, IFN at the same concentration inhibited both IgG and IgA production by PB MNCs in healthy controls. In studying BM cells, IFN was found to reduce IgA production in IgA-MM, but had a neutral effect on IgG production in IgG-MM. In the controls, the production of both the IgG and the IgA isotypes by BM MNCs was decreased by IFN. On the basis of these results it seems that the disease itself somehow affects the Ig-producing cells in MM, when measured as different responses of the cells to exogenous IFN in vitro. The results do not support the hypothesis that IFN treatment could restore normal Ig production in MM patients.
为了验证α干扰素（IFN）治疗可能恢复多发性骨髓瘤（MM）患者正常免疫球蛋白（Ig）产生这一假设，通过酶联免疫吸附测定（ELISA）分析了IFN对MM患者外周血（PB）和骨髓（BM）单个核细胞（MNCs）产生Ig同种型（IgG和IgA）的影响。发现浓度为1000 U/ml的IFN可增强IgA-MM患者PB MNCs的IgA产生，并在IgG-MM患者中有刺激IgG产生的趋势。IFN对非副蛋白Ig同种型产生的影响更具变异性，在两种MM亚型中大多观察到中性或抑制作用。与在MM患者中观察到的影响相反，相同浓度的IFN抑制健康对照者PB MNCs的IgG和IgA产生。在研究BM细胞时，发现IFN可降低IgA-MM患者的IgA产生，但对IgG-MM患者的IgG产生具有中性作用。在对照中，IFN降低了BM MNCs的IgG和IgA同种型的产生。基于这些结果，当以细胞在体外对外源性IFN的不同反应来衡量时，似乎疾病本身以某种方式影响了MM患者的Ig产生细胞。这些结果不支持IFN治疗可恢复MM患者正常Ig产生这一假设。
