Reivinen J, Holthöfer H, Miettinen A
Department of Bacteriology and Immunology, University of Helsinki, Finland.
Int Immunol. 1994 Sep;6(9):1409-16. doi: 10.1093/intimm/6.9.1409.
O-acetyl GD3 ganglioside is a cell surface molecule of some neural, neural crest and renal cells. Here we show, by using mAbs specific for O-acetyl GD3 (clone 27A) and flow-cytometric, biochemical or immunological techniques, that it is also expressed at high intensity level on the surface of 49.6% (median) of the CD3+ cells (T lymphocytes), at medium level in 16.2% of the CD16+ (natural killer) cells, at very low level in 51.9% of CD14+ cells (monocytes) and in 6.9% of CD20+ cells (B lymphocytes), but not in other human blood cells. Of the CD4+ or CD8+ cells, 52.6 or 36.5% respectively were 27A+. Furthermore, 81.6% of the CD45RO+ lymphocytes carried the O-acetyl GD3 ganglioside. It was not detected in the thymus, although its immediate precursor, the GD3 ganglioside, was present in the medullary thymocytes, suggesting that O-acetyltransferases are regulated by maturation events taking place in the periphery. The anti-O-acetyl GD3 antibodies induced a strong mitogenic response in cultured peripheral blood mononuclear cells, but not in purified T cells. However, in combination with phorbol myristate acetate the antibodies induced proliferation also in purified T cells, suggesting that protein kinase C priming is needed for this effect. This and the restricted expression of O-acetyl GD3 suggest a functional role for this ganglioside in T cell subpopulations.
O-乙酰神经节苷脂GD3是一些神经细胞、神经嵴细胞和肾细胞的细胞表面分子。在此我们通过使用对O-乙酰GD3特异的单克隆抗体（克隆27A）以及流式细胞术、生化或免疫学技术表明，它也在49.6%（中位数）的CD3⁺细胞（T淋巴细胞）表面高强度表达，在16.2%的CD16⁺（自然杀伤）细胞中中等强度表达，在51.9%的CD14⁺细胞（单核细胞）和6.9%的CD20⁺细胞（B淋巴细胞）中极低强度表达，但在其他人类血细胞中不表达。在CD4⁺或CD8⁺细胞中，分别有52.6%或36.5%为27A⁺阳性。此外，81.6%的CD45RO⁺淋巴细胞携带O-乙酰神经节苷脂GD3。在胸腺中未检测到它，尽管其直接前体神经节苷脂GD3存在于胸腺髓质细胞中，这表明O-乙酰转移酶受外周发生的成熟事件调控。抗O-乙酰GD3抗体在培养的外周血单个核细胞中诱导强烈的促有丝分裂反应，但在纯化的T细胞中不诱导。然而，与佛波酯肉豆蔻酸酯联合使用时，这些抗体也能诱导纯化T细胞增殖，表明这种效应需要蛋白激酶C启动。O-乙酰GD3的这种表达受限情况提示该神经节苷脂在T细胞亚群中具有功能作用。
