Kniep B, Claus C, Peter-Katalinic J, Monner D A, Dippold W, Nimtz M
GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Federal Republic of Germany.
J Biol Chem. 1995 Dec 15;270(50):30173-80. doi: 10.1074/jbc.270.50.30173.
The monoclonal antibody U5, which is a potent inducer of proliferation in human T-cells, was found to bind to an alkali-sensitive derivative of ganglioside GD3. Using immunochemical and spectroscopic methods, the structure of the U5 antigen was determined as 7-O-acetyl-GD3. The antibody U5 did not react with 9-O-acetyl-GD3 and bound severalfold more stronger to 7-O-acetyl-GD3 than to GD3. U5 is the first antibody known to detect preferentially 7-O-acetyl-GD3. Flow cytometric analysis showed that each major class of human leukocytes contained a significant fraction of cells binding the U5 antibody.
单克隆抗体U5是人类T细胞增殖的有效诱导剂，被发现可与神经节苷脂GD3的碱敏感衍生物结合。通过免疫化学和光谱学方法，确定U5抗原的结构为7-O-乙酰基-GD3。抗体U5不与9-O-乙酰基-GD3反应，且与7-O-乙酰基-GD3的结合力比对GD3的结合力强几倍。U5是已知的第一种优先检测7-O-乙酰基-GD3的抗体。流式细胞术分析表明，每一类主要的人类白细胞中都有相当一部分细胞与U5抗体结合。
