Itzhak Y, Norenberg M D
Department of Biochemistry and Molecular Biology (R-629), University of Miami School of Medicine, FL 33101.
Brain Res. 1994 Oct 17;660(2):346-8. doi: 10.1016/0006-8993(94)91311-0.
Exposure of primary cultured astrocytes for 3 days to 1 microM of either dopamine, serotonin or norepinephrine resulted in upregulation (25-34% increase in Bmax) of the peripheral-type benzodiazepine receptors (PBRs) labeled with [3H]Ro5-4864. A similar treatment with gamma-aminobutyric acid [GABA] caused a 2-fold increase in the affinity (Kd) of [3H]Ro5-4864. The monoamines tested and GABA had no effect on the binding parameters of [3H]PK 11195, another selective PBR ligand. The present study indicates that Ro5-4864 binding sites are susceptible to regulation by specific neurotransmitters and provides further evidence for the distinction between Ro5-4864 and PK 11195 binding sites of the PBRs in cultured astrocytes.
将原代培养的星形胶质细胞分别暴露于1微摩尔的多巴胺、血清素或去甲肾上腺素3天，会导致用[3H]Ro5 - 4864标记的外周型苯二氮䓬受体（PBRs）上调（Bmax增加25 - 34%）。用γ-氨基丁酸[GABA]进行类似处理会使[3H]Ro5 - 4864的亲和力（Kd）增加2倍。所测试的单胺类物质和GABA对另一种选择性PBR配体[3H]PK 11195的结合参数没有影响。本研究表明，Ro5 - 4864结合位点易受特定神经递质的调节，并为培养的星形胶质细胞中PBRs的Ro5 - 4864和PK 11195结合位点之间的差异提供了进一步的证据。
