氯化铵对星形胶质细胞苯二氮䓬受体的影响。

Effect of ammonium chloride on the astrocyte benzodiazepine receptor.

作者信息

Ducis I, Norenberg L O, Norenberg M D

机构信息

Department of Pathology, University of Miami/Jackson Memorial Hospital, FL.

出版信息

Brain Res. 1989 Jul 31;493(2):362-5. doi: 10.1016/0006-8993(89)91171-2.

DOI:10.1016/0006-8993(89)91171-2

PMID:2548671

Abstract

We have carried out studies on the effect of ammonium chloride on the astrocyte benzodiazepine receptor. Scatchard analysis of the binding of [3H]Ro-5-4864 to homogenates prepared from primary astrocyte cultures showed a significant decrease in Kd (27% with 2 mM NH4Cl; 32% with 5 mM NH4Cl; 25% with 10 mM NH4Cl) and Bmax (14% with 10 mM NH4Cl). These findings indicate that ammonium chloride can affect the astrocyte benzodiazepine receptor, and that such receptor changes may contribute to ammonia-induced encephalopathy.

摘要

我们已经开展了关于氯化铵对星形胶质细胞苯二氮䓬受体影响的研究。对从原代星形胶质细胞培养物制备的匀浆进行的[3H]Ro-5-4864结合的Scatchard分析显示，解离常数（Kd）显著降低（2 mM氯化铵时降低27%；5 mM氯化铵时降低32%；10 mM氯化铵时降低25%），最大结合容量（Bmax）也降低（10 mM氯化铵时降低14%）。这些发现表明，氯化铵可影响星形胶质细胞苯二氮䓬受体，且这种受体变化可能与氨诱导的肝性脑病有关。