Noda N, Tanaka R, Tsujino K, Takasaki Y, Nakano M, Nishi M, Miyahara K
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka.
J Biochem. 1994 Aug;116(2):435-42. doi: 10.1093/oxfordjournals.jbchem.a124543.
Seven glycosphingolipids were obtained in the pure state from the clam worm, Marphysa sanguinea, by preparative HPLC with a reversed-phase column in a recycling mode. Their structures were elucidated based on detailed analyses of 1H- and 13C-NMR spectra of the intact compounds. The position and the geometry of double bonds in the long-chain base (LCB) were determined by the two-dimensional NMR (COSY, NOESY, and HMBC) analyses. All compounds are phosphocholine-bonded monogalactosylceramides and two of them have tri-unsaturated long-chain bases.
通过使用反相柱以循环模式进行制备型高效液相色谱法，从沙蚕（Marphysa sanguinea）中获得了七种纯态的糖鞘脂。基于对完整化合物的¹H-和¹³C-NMR光谱的详细分析，阐明了它们的结构。通过二维NMR（COSY、NOESY和HMBC）分析确定了长链碱（LCB）中双键的位置和几何形状。所有化合物均为磷酸胆碱键合的单半乳糖基神经酰胺，其中两种具有三不饱和长链碱。
