• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

单日载脂蛋白E基因分型

Single-day apolipoprotein E genotyping.

作者信息

Crook R, Hardy J, Duff K

机构信息

Suncoast Alzheimer's Disease Laboratories, Department of Psychiatry, University of South Florida, Tampa 33613.

出版信息

J Neurosci Methods. 1994 Aug;53(2):125-7. doi: 10.1016/0165-0270(94)90168-6.

DOI:10.1016/0165-0270(94)90168-6
PMID:7823614
Abstract

Current methods of genotyping an individual's apolipoprotein (ApoE) alleles involve time-consuming separations of digested fragments on high-percentage non-denaturing polyacrylamide gels. However, it is possible to separate the fragments quicker and with greater ease using agarose.

摘要

目前对个体载脂蛋白(ApoE)等位基因进行基因分型的方法,涉及在高百分比非变性聚丙烯酰胺凝胶上对消化后的片段进行耗时的分离。然而,使用琼脂糖可以更快速、更轻松地分离这些片段。

相似文献

1
Single-day apolipoprotein E genotyping.单日载脂蛋白E基因分型
J Neurosci Methods. 1994 Aug;53(2):125-7. doi: 10.1016/0165-0270(94)90168-6.
2
Apolipoprotein E2/E3/E4 genotyping with agarose gels.使用琼脂糖凝胶进行载脂蛋白E2/E3/E4基因分型。
Clin Chem. 1995 Jan;41(1):73-5.
3
Apolipoprotein E genotyping on agarose gels.
Clin Chem. 1995 Jul;41(7):1046-7.
4
Concept of VNTR alleles: comparison of apolipoprotein B 3' hypervariable region genotyping results obtained by three methods.可变数目串联重复序列(VNTR)等位基因的概念:三种方法获得的载脂蛋白B 3'高变区基因分型结果比较
Biochem Biophys Res Commun. 1991 Dec 16;181(2):846-51. doi: 10.1016/0006-291x(91)91267-g.
5
Capillary electrophoretic detection in apolipoprotein E genotyping.载脂蛋白E基因分型中的毛细管电泳检测
Electrophoresis. 2002 Jun;23(12):1878-81. doi: 10.1002/1522-2683(200206)23:12<1878::AID-ELPS1878>3.0.CO;2-G.
6
Apolipoprotein E genotyping using polymerase chain reaction.使用聚合酶链反应进行载脂蛋白E基因分型。
N Z Med J. 1990 Feb 28;103(884):80-1.
7
Apolipoprotein E genotyping methods for the clinical laboratory.
J Clin Lab Anal. 1995;9(1):63-9. doi: 10.1002/jcla.1860090112.
8
Analysis of apolipoprotein E genotypes by the Amplification Refractory Mutation System.
Clin Chem. 1991 Feb;37(2):241-4.
9
Improved method for genotyping apolipoprotein E polymorphisms by a PCR-based assay simultaneously utilizing two distinct restriction enzymes.一种基于聚合酶链反应(PCR)的检测方法,通过同时使用两种不同的限制性内切酶来改进载脂蛋白E基因多态性的基因分型方法。
Clin Chem. 1997 Sep;43(9):1657-9.
10
Detection of human apolipoprotein E3, E2, and E4 genotypes by an allele-specific oligonucleotide-primed polymerase chain reaction assay: development and validation.通过等位基因特异性寡核苷酸引物聚合酶链反应分析检测人类载脂蛋白E3、E2和E4基因型:方法建立与验证
Clin Chem. 1991 Jul;37(7):1263-8.

引用本文的文献

1
Associations of Chronic Insomnia, Longitudinal Cognitive Outcomes, Amyloid-PET, and White Matter Changes in Cognitively Normal Older Adults.认知正常的老年人中慢性失眠、纵向认知结果、淀粉样蛋白PET和白质变化的关联
Neurology. 2025 Oct 7;105(7):e214155. doi: 10.1212/WNL.0000000000214155. Epub 2025 Sep 10.
2
F-FDG PET in detection of primary age-related tauopathy (PART) - Is there a role? Insights from an imaging-pathology correlation study.F-FDG PET在原发性年龄相关性tau蛋白病(PART)检测中的作用——是否有作用?一项影像-病理相关性研究的见解
Alzheimers Dement. 2025 Aug;21(8):e70568. doi: 10.1002/alz.70568.
3
Aβ and p-tau 181 as disease biomarkers in atypical Alzheimer's disease.
β淀粉样蛋白和磷酸化tau蛋白181作为非典型阿尔茨海默病的疾病生物标志物。
J Alzheimers Dis. 2025 Jun;105(3):923-931. doi: 10.1177/13872877251333450. Epub 2025 Apr 23.
4
Platelets mirror changes in the frontal lobe antioxidant system in Alzheimer's disease.血小板反映了阿尔茨海默病额叶抗氧化系统的变化。
Alzheimers Dement. 2025 Apr;21(4):e70117. doi: 10.1002/alz.70117.
5
Relation of Alzheimer's disease-related TDP-43 proteinopathy to metrics from diffusion tensor imaging (DTI) and neurite orientation dispersion and density imaging (NODDI).阿尔茨海默病相关的TDP-43蛋白病与扩散张量成像(DTI)及神经突方向离散度和密度成像(NODDI)指标的关系。
Neurobiol Aging. 2025 Jun;150:97-108. doi: 10.1016/j.neurobiolaging.2025.03.001. Epub 2025 Mar 8.
6
White matter hyperintensities in dementia with lewy bodies and posterior cortical atrophy.路易体痴呆和后皮质萎缩中的白质高信号
Neurobiol Aging. 2025 Jun;150:44-52. doi: 10.1016/j.neurobiolaging.2025.03.002. Epub 2025 Mar 3.
7
Non-rapid eye movement sleep slow-wave activity features are associated with amyloid accumulation in older adults with obstructive sleep apnoea.非快速眼动睡眠慢波活动特征与患有阻塞性睡眠呼吸暂停的老年人淀粉样蛋白积累有关。
Brain Commun. 2024 Oct 7;6(5):fcae354. doi: 10.1093/braincomms/fcae354. eCollection 2024.
8
Investigating the feasibility of F-flortaucipir PET imaging in the antemortem diagnosis of primary age-related tauopathy (PART): An observational imaging-pathological study.F-氟代托品PET成像在原发性年龄相关性tau蛋白病(PART)生前诊断中的可行性研究:一项观察性影像病理学研究。
Alzheimers Dement. 2024 Dec;20(12):8605-8614. doi: 10.1002/alz.14301. Epub 2024 Oct 17.
9
Exploring first-degree family history in a cohort of Portuguese Alzheimer's disease patients: population evidence for X-chromosome linked and recessive inheritance of risk factors.在一组葡萄牙阿尔茨海默病患者中探究一级家族史:X染色体连锁和隐性危险因素遗传的群体证据。
J Neurol. 2024 Oct;271(10):6983-6990. doi: 10.1007/s00415-024-12673-x. Epub 2024 Sep 5.
10
Flortaucipir PET uncovers relationships between tau and amyloid-β in primary age-related tauopathy and Alzheimer's disease.Flortaucipir PET 揭示了原发性年龄相关性 tau 病和阿尔茨海默病中 tau 和淀粉样蛋白-β 之间的关系。
Sci Transl Med. 2024 Jul 24;16(757):eado8076. doi: 10.1126/scitranslmed.ado8076.