Heinlein M
Institut für Genetik, University of Cologne, Germany.
Mol Gen Genet. 1995 Jan 6;246(1):1-9. doi: 10.1007/BF00290127.
The Ac elements present in the unstable wx-m7 and wx-m9 alleles of maize trigger different patterns of Ds excision in trans. To determine whether this differential regulation is a feature of the Ac alleles themselves or is mediated by genetically distinct factors, maize plants heterozygous for the wx-m7 and wx-m9 alleles were crossed to tester strains homozygous for Ds reporter alleles. Kernels showing the variegation pattern characteristic for the Ac elements carried in the wx-m7 and wx-m9 alleles were found to be present in the ratios expected from the genetic constitution of the strains. The aleurone variegation caused by excision of the Ds reporter element and the endosperm variegation caused by excision of Ac from the wx-m7 and wx-m9 alleles themselves segregated with the original wx-m alleles. In addition, stable Wx and wx derivatives of wx-m9 that have lost Ac no longer exert any trans effect on the wx-m7 allele (and vice versa). Therefore it is concluded that the observed variegation patterns are autonomously determined by specific trans effects of the particular Ac element.
玉米不稳定的wx - m7和wx - m9等位基因中存在的Ac元件会引发不同模式的Ds反式切除。为了确定这种差异调控是Ac等位基因自身的特性，还是由基因上不同的因子介导的，将携带wx - m7和wx - m9等位基因的杂合玉米植株与携带Ds报告基因等位基因的纯合测试菌株进行杂交。结果发现，呈现出wx - m7和wx - m9等位基因中携带的Ac元件特征性斑驳模式的籽粒，其比例符合菌株遗传组成预期。Ds报告元件切除引起的糊粉层斑驳以及wx - m7和wx - m9等位基因自身的Ac切除引起的胚乳斑驳，都与原始的wx - m等位基因一起分离。此外，已失去Ac的wx - m9的稳定Wx和wx衍生物不再对wx - m7等位基因产生任何反式效应（反之亦然）。因此得出结论，观察到的斑驳模式是由特定Ac元件的特定反式效应自主决定的。
